Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere

Stable Identifier
R-HSA-174439
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere
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The binding of the primer recognition complex involves the loading of the proliferating cell nuclear antigen (PCNA). Replication Factor C (RFC) transiently opens the PCNA toroid in an ATP-dependent reaction, and then allows PCNA to re-close around the double helix adjacent to the primer terminus. This leads to the formation of the "sliding clamp" (Tsurimoto et al. 1990, Mossi and Hubscher 1998). In a human telomere replication model, RFC-mediated PCNA loading increases the processivity of telomeric C-strand synthesis, but does not eliminate polymerase delta stalling on the G-rich template (Lormand et al. 2013).
Interaction of RTEL1 with PCNA is needed for telomere replication and maintenance of telomere integrity (Vannier et al. 2013).
Literature References
PubMed ID Title Journal Year
24115439 RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication

Vannier, JB, Sandhu, S, Petalcorin, MI, Wu, X, Nabi, Z, Ding, H, Boulton, SJ

Science 2013
24038470 DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation

Lormand, JD, Buncher, N, Murphy, CT, Kaur, P, Lee, MY, Burgers, P, Wang, H, Kunkel, TA, Opresko, PL

Nucleic Acids Res. 2013
Participants
Input
Output
Participates
as an event of
Event Information
Go Biological Process
telomere maintenance via semi-conservative replication (0032201)
Catalyst Activity

DNA clamp loader activity of RFC Heteropentamer:RNA primer-DNA primer:G-strand extended telomere end [nucleoplasm]

Physical Entity
RFC Heteropentamer:RNA primer-DNA primer:G-strand extended telomere end [nucleoplasm] (Homo sapiens)
Activity
DNA clamp loader activity (GO:0003689)
Orthologous Events
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Bos taurus)
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Canis familiaris)
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Gallus gallus)
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Mus musculus)
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Rattus norvegicus)
Loading of PCNA - Sliding Clamp Formation on the C-strand of the telomere (Sus scrofa)
Authored
Blackburn, EH  (2006-03-10)
Seidel, J  (2006-03-10)
Reviewed
Price, C  (2006-07-13)
Hayashi, MT  (2020-04-29)
Created
Gillespie, ME  (2006-02-18)
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