Formation of C-strand Okazaki fragments

Stable Identifier
R-HSA-174444
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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After RFC initiates the assembly of the primer recognition complex, the complex of pol delta and PCNA is responsible for incorporating the additional nucleotides prior to the position of the next downstream initiator RNA primer. On the lagging strand, short discontinuous segments of DNA, called Okazaki fragments, are synthesized on RNA primers. The average length of the Okazaki fragments is 100 nucleotides. Polymerase switching is a key event that allows the processive synthesis of DNA by the pol delta and PCNA complex (Lee and Hurwitz 1990, Tsurimoto and Stillman 1991, Nethanel et al. 1992, Brown and Campbell 1993, Waga et al.1994, Bambara et al. 1997). PCNA increases the processivity of the DNA polymerase delta during telomeric C-strand synthesis in a human telomere replication model, but it does not eliminate the DNA polymerase delta stalling on the G-rich template (Lormand et al. 2013).

Literature References
PubMed ID Title Journal Year
24038470 DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation

Lormand, JD, Buncher, N, Murphy, CT, Kaur, P, Lee, MY, Burgers, P, Wang, H, Kunkel, TA, Opresko, PL

Nucleic Acids Res. 2013
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Event Information
Catalyst Activity

DNA-directed DNA polymerase activity of Processive complex loaded on telomere [nucleoplasm]

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