Removal of remaining Flap from the C-strand

Stable Identifier
R-HSA-174446
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

The remaining flap, which is too short to support RPA binding, is then processed by FEN-1. There is evidence that binding of RPA to the displaced end of the RNA-containing Okazaki fragment prevents FEN-1 from accessing the substrate. FEN-1 is a structure-specific endonuclease that cleaves near the base of the flap at a position one nucleotide into the annealed region. Biochemical studies have shown that the preferred substrate for FEN-1 consists of a one-nucleotide 3'-tail on the upstream primer in addition to the 5'-flap of the downstream primer.

Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
5'-flap endonuclease activity of FEN1 [nucleoplasm]
Physical Entity
Activity
Orthologous Events