Reactome | HUS1 [nucleoplasm]

HUS1 [nucleoplasm]

Stable Identifier

R-HSA-176374

Type

Protein [EntityWithAccessionedSequence]

Species

Homo sapiens

Compartment

nucleoplasm

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Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle Checkpoints (Homo sapiens)
  - G2/M Checkpoints (Homo sapiens)
    - Activation of ATR in response to replication stress (Homo sapiens)
      - Recruitment of the Rad9-Hus1-Rad1 complex to DNA (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        Rad9-Hus1-Rad1 bound to DNA [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
    - G2/M DNA damage checkpoint (Homo sapiens)
      - ATR phosphorylates TP53 (Homo sapiens)
        
        ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)

DNA Repair (Homo sapiens)

- DNA Double-Strand Break Repair (Homo sapiens)
  - Homology Directed Repair (Homo sapiens)
    - HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) (Homo sapiens)
      - HDR through Homologous Recombination (HRR) (Homo sapiens)
        
        Homologous DNA Pairing and Strand Exchange (Homo sapiens)
        
        Presynaptic phase of homologous DNA pairing and strand exchange (Homo sapiens)
        
        RAD51 binds BRCA2 at resected DNA DSBs (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
      - HDR through Single Strand Annealing (SSA) (Homo sapiens)
        
        Association of RAD51 with RAD52:DNA double-strand break ends (Homo sapiens)
        
        RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        Association of RAD52 with the RPA complex at resected DNA DSBs (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        ERCC1:XPF cleaves flaps generated by SSA (Homo sapiens)
        
        RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        RAD52 promotes single strand annealing at resected DNA DSBs (Homo sapiens)
        
        RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        RAD51:p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        p-Y104-RAD52:p-RPA:ATR:ATRIP:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:DNA DSBs with annealed 3' ssDNA overhangs and displaced flaps:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
      - Processing of DNA double-strand break ends (Homo sapiens)
        
        ATR activation at DNA DSBs (Homo sapiens)
        
        ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        ATR phosphorylates RPA2 (Homo sapiens)
        
        ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        Activation of CHEK1 at resected DNA DSBs (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1:TIMELESS:TIPIN:p-T916,S945-CLSPN:CHEK1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        CHEK1 is recruited to resected DNA DSBs (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1:TIMELESS:TIPIN:p-T916,S945-CLSPN:CHEK1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)

Disease (Homo sapiens)

- Diseases of DNA repair (Homo sapiens)
  - Diseases of DNA Double-Strand Break Repair (Homo sapiens)
    - Defective homologous recombination repair (HRR) due to BRCA2 loss of function (Homo sapiens)
      - Impaired BRCA2 binding to RAD51 (Homo sapiens)
        
        BRCA2 mutants with BRC defects or a defect in the C-terminal RAD51 binding site do not bind RAD51 (Homo sapiens)
        
        ATR:ATRIP:p-RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)

Gene expression (Transcription) (Homo sapiens)

- RNA Polymerase II Transcription (Homo sapiens)
  - Generic Transcription Pathway (Homo sapiens)
    - Transcriptional Regulation by TP53 (Homo sapiens)
      - Regulation of TP53 Activity (Homo sapiens)
        
        Regulation of TP53 Activity through Phosphorylation (Homo sapiens)
        
        ATR phosphorylates TP53 (Homo sapiens)
        
        ATR:ATRIP:RPA:3' overhanging ssDNA-DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:BRCA1-C complex:EXO1,DNA2:BLM,WRN:p-S990,Ac-K1249-BRIP1:RAD17:RFC:RAD9:HUS1:RAD1:RHNO1:TOPBP1 [nucleoplasm] (Homo sapiens)
        
        RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)
        
        HUS1 [nucleoplasm] (Homo sapiens)

External Reference Information

External Reference

UniProt:O60921 HUS1

Gene Names

HUS1

Chain

chain:1-280

Reference Genes

BioGPS Gene:3364 HUS1

COSMIC (genes):HUS1 HUS1

CTD Gene:3364 HUS1

dbSNP Gene:3364 HUS1

ENSEMBL:ENSG00000136273 HUS1

ENSEMBL_homo_sapiens_GENE:ENSG00000136273.13 HUS1

HGNC:5309 HUS1

KEGG Gene (Homo sapiens):3364 HUS1

Monarch:3364 HUS1

NCBI Gene:3364 HUS1

OMIM:603760 HUS1

UCSC:O60921 HUS1

Reference Transcript

Other Identifiers

11739311_a_at

11739312_a_at

11746197_a_at

17057579

204883_PM_s_at

204883_s_at

204884_3p_s_at

204884_PM_s_at

204884_s_at

217618_3p_x_at

217618_PM_x_at

217618_x_at

3049843

3049844

3049845

3049846

3049847

3049848

3049849

3049850

3049852

3049855

3049858

3049859

3049861

3049862

3049863

3049864

3049865

3049866

3049867

3364

38153_at

80356_r_at

80446_at

8139592

A_23_P156977

A_24_P932706

A_33_P3330841

GE54702

GO:0000077

GO:0000228

GO:0000278

GO:0000723

GO:0000724

GO:0001932

GO:0005515

GO:0005634

GO:0005654

GO:0005694

GO:0005730

GO:0005737

GO:0005829

GO:0006281

GO:0006289

GO:0006310

GO:0006974

GO:0009411

GO:0009792

GO:0022414

GO:0023052

GO:0030896

GO:0031573

GO:0032200

GO:0033314

GO:0035861

GO:0036211

GO:0043226

GO:0044778

GO:0048856

GO:0071479

HMNXSV003019403

HMNXSV003031782

Hs.152983.0.S1_3p_a_at

Hs.233299.0.A1_3p_x_at

ILMN_1670462

ILMN_2136615

PH_hs_0009200

TC07001364.hg

TC07002933.hg

g4758575_3p_a_at

Participates

as a component of

RAD9:HUS1:RAD1 [nucleoplasm] (Homo sapiens)

Inferred To

HUS1 [nucleoplasm] (Bos taurus)

HUS1 [nucleoplasm] (Canis familiaris)

HUS1 [nucleoplasm] (Gallus gallus)

HUS1 [nucleoplasm] (Sus scrofa)

LOC119865601 [nucleoplasm] (Canis familiaris)

Hus1-like [nucleoplasm] (Drosophila melanogaster)

hus-1 [nucleoplasm] (Caenorhabditis elegans)

Homologues of HUS1 [nucleoplasm] (Canis familiaris)

MEC3 [nucleoplasm] (Saccharomyces cerevisiae)

Hus1 [nucleoplasm] (Mus musculus)

Hus1 [nucleoplasm] (Rattus norvegicus)

hus1 [nucleoplasm] (Dictyostelium discoideum)

hus1 [nucleoplasm] (Schizosaccharomyces pombe)

hus1 [nucleoplasm] (Xenopus tropicalis)

Cross References

RefSeq

XP_016867604.1, NP_004498.1

OpenTargets

ENSG00000136273

GeneCards

O60921

HPA

ENSG00000136273-HUS1

Ensembl

ENST00000258774, ENSG00000136273, ENST00000432325, ENSP00000416588, ENSP00000258774, ENST00000458191, ENSP00000400727

PRO

O60921

Pharos - Targets

O60921

PDB

6J8Y, 3GGR, 3G65, 8GNN, 7Z6H, 3A1J

Interactors (1)

Accession	#Entities	Entities	Confidence Score	Evidence (IntAct)
UniProt:O00560 SDCBP	3	SDCBP [extracellular region] (R-HSA-6806206) SDCBP [azurophil granule lumen] (R-HSA-6799372) SDCBP [cytosol] (R-HSA-373639)	0.556	3

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