Newly synthesized vRNP for export

Stable Identifier
R-HSA-192677
Type
Reaction
Species
Homo sapiens
Related Species
Influenza A virus
Compartment
nucleoplasm
Locations in the PathwayBrowser
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Summation

The nascent vRNP complexes, one for each gene segment, contain the negative-sense viral RNA and polymerase proteins (PB1, PB2, PA, and NP). In a model using negative-sense viral RNP reconstituted from transfected cells, there are multiple NP complexes and one polymerase complex arranged along a closed vRNA loop (Area et al., 2004). The three-dimensional structure of NP has revealed that three NP molecules form a stable trimer, interacting through beta-sheets b5, b6, and b7 in the C-terminal domain of the protein (Ye, 2006), with the viral RNA wrapping around the outside of the complex. Viral RNA from purified virions is present in an RNase-sensitive complex with NP and PB1, PB2, and PA, consistent with this structural model (Baudin et al, 1994; Ruigrok et al., 1995; Klumpp et al., 1997). It is not clear what controls the fate of vRNP, whether it is destined to become a template for transcription, for replication, or for export into the cytoplasm for packaging into virions at the plasma membrane, nor how distinct sub-nuclear localization and NP distribution at the nuclear matrix might mark, or polarize, a vRNP for export (Elton, 2005; Takizawa et al., 2006).

Literature References
PubMed ID Title Journal Year
14691253 3D structure of the influenza virus polymerase complex: localization of subunit domains

Area, E, Martin-Benito, J, Gastaminza, P, Torreira, E, Valpuesta, JM, Carrascosa, JL, Ortin, J

Proc Natl Acad Sci U S A 2004
17151603 The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA

Ye, Q, Krug, RM, Tao, YJ

Nature 2006
Participants
Input
Output
Participant Of
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This entity is regulated by
Positive Regulation
Disease
Name Identifier Synonyms
influenza 8469 Influenza with other manifestations (disorder), Influenza with other manifestations NOS (disorder), flu, Influenza with other manifestations, Influenza with non-respiratory manifestation (disorder), influenza with non-respiratory manifestation
Authored
Bortz, E  (2007-02-13)
Garcia-Sastre, A  (2007-02-13)
Reviewed
Squires, B  (2007-02-13)