CYP51A1 demethylates LNSOL

Stable Identifier
R-HSA-194678
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
lanosterol + 3 NADPH + 3 H+ + 3 O2 => 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + 3 NADP+ + 4 H2O + formate
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Lanosterol 14-alpha demethylase (CYP51A1) catalyses oxidative C14-demethylation of lanosterol (LNSOL) to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol (4,4DMCHOLtrienol). Although the reaction is annotated here as a single concerted event, studies with purified rat enzyme indicate that the methyl group is converted successively to an alcohol and an aldehyde before being released as formate (Stromstedt et al. 1996, Strushkevich et al. 2010).

Literature References
PubMed ID Title Journal Year
8619637 The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols

Stromstedt, M, Rozman, D, Waterman, MR

Arch Biochem Biophys 1996
20149798 Structural basis of human CYP51 inhibition by antifungal azoles

Strushkevich, N, Usanov, SA, Park, HW

J. Mol. Biol. 2010
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
sterol 14-demethylase activity of CYP51A1 [endoplasmic reticulum membrane]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created