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2xTPK1:Mg2+ phosphorylates THMN

Stable Identifier
R-HSA-196761
Type
Reaction
Species
Homo sapiens
Compartment
Synonyms
Thiamin is pyrophosphorylated
Locations in the PathwayBrowser
Summation

Cytosolic thiamin pyrophosphokinase (TPK1) catalyzes the reaction of thiamin (THMN) and ATP to form thiamin diphosphate (ThDP aka thiamin pyrophosphate) and ADP. ThDP is an active cofactor for transketolase, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, enzymes involved in glycolysis and energy production. The gene encoding the human enzyme has been cloned and its protein product has been shown to have TPK1 activity (Nosaka et al. 2001; Zhao et al. 2001). Its homodomeric structure and association with Mg2+ are inferred from properties of the homologous yeast enzyme (Baker et al. 2001).

Literature References
PubMed ID Title Journal Year
11342111 Isolation and characterization of a human thiamine pyrophosphokinase cDNA Biochim Biophys Acta 2001
11342117 Molecular cloning of human thiamin pyrophosphokinase Biochim Biophys Acta 2001
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
thiamine diphosphokinase activity of 2xTPK1:Mg2+ [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea