Reactome: A Curated Pathway Database

THF polyglutamate + formate + ATP => 10-formylTHF polyglutamate + ADP + orthophosphate

Stable Identifier
R-HSA-200711
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

The formate-tetrahydrofolate ligase activity of the trifunctional MTHFD1 enzyme catalyzes the reaction of THF polyglutamate, formate, and ATP to form 10-formylTHF polyglutamate, ADP, and orthophosphate. MTHFD1 is cytosolic and occurs as a dimer. The human enzyme has been identified and partially characterized biochemically (Hum et al. 1988); additional reaction details can be inferred from the properties of the well-studied homologous rabbit enzyme (Villar et al. 1985).

Literature References
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
formate-tetrahydrofolate ligase activity of 2xMTHFD1 [cytosol] 2xMTHFD1 [cytosol] formate-tetrahydrofolate ligase activity (0004329)
Orthologous Events