PRC2 trimethylates histone H3 at lysine-27

Stable Identifier
R-HSA-212263
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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EZH2 , a SET domain protein, trimethylates histone H3 at lysine 27 (H3K27) and lysine 9 (H3K9) (Kuzmichev et al. 2002, Cao et al. 2002, Kuzmichev et al. 2004, Martin et al. 2006, McCabe et al. 2012, Swalm et al. 2013). EZH2 has greater activity with lysine residues that have fewer methyl groups (unmethylated:monomethylated:dimethylated=9:6:1, McCabe et al. 2012). EZH1 rather than EZH2 is present in some PRC2 complexes and can also catalyze the trimethylation of H3K27 (inferred from mouse in Shen et al. 2008), however EZH1 is also able to repress transcription without methylating histone H3 (Margueron et al. 2008).

Literature References
PubMed ID Title Journal Year
22323599 Mutation of A677 in histone methyltransferase EZH2 in human B-cell lymphoma promotes hypertrimethylation of histone H3 on lysine 27 (H3K27)

McCabe, MT, Graves, AP, Ganji, G, Diaz, E, Halsey, WS, Jiang, Y, Smitheman, KN, Ott, HM, Pappalardi, MB, Allen, KE, Chen, SB, Della Pietra, A, Dul, E, Hughes, AM, Gilbert, SA, Thrall, SH, Tummino, PJ, Kruger, RG, Brandt, M, Schwartz, B, Creasy, CL

Proc. Natl. Acad. Sci. U.S.A. 2012
15099518 Different EZH2-containing complexes target methylation of histone H1 or nucleosomal histone H3

Kuzmichev, A, Jenuwein, T, Tempst, P, Reinberg, D

Mol Cell 2004
12351676 Role of histone H3 lysine 27 methylation in Polycomb-group silencing

Cao, R, Wang, L, Wang, H, Xia, L, Erdjument-Bromage, H, Tempst, P, Jones, RS, Zhang, Y

Science 2002
23679895 Convergent evolution of chromatin modification by structurally distinct enzymes: comparative enzymology of histone H3 Lys²? methylation by human polycomb repressive complex 2 and vSET

Swalm, BM, Hallenbeck, KK, Majer, CR, Jin, L, Scott, MP, Moyer, MP, Copeland, RA, Wigle, TJ

Biochem. J. 2013
12435631 Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein

Kuzmichev, A, Nishioka, K, Erdjument-Bromage, H, Tempst, P, Reinberg, D

Genes Dev 2002
16431907 Substrate preferences of the EZH2 histone methyltransferase complex

Martin, C, Cao, R, Zhang, Y

J Biol Chem 2006
19026781 Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms

Margueron, R, Li, G, Sarma, K, Blais, A, Zavadil, J, Woodcock, CL, Dynlacht, BD, Reinberg, D

Mol. Cell 2008
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein-lysine N-methyltransferase activity of PRC2:Chromatin [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Reviewed
Created