carbovir diphosphate + ATP => carbovir triphosphate + ADP

Stable Identifier
R-HSA-2162096
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
Synonyms
Phosphorylation of carbovir diphosphate
ReviewStatus
5/5
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carbovir diphosphate + ATP => carbovir triphosphate + ADP
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Cytosolic PCK1 (phosphoenolpyruvate carboxykinase 1) catalyzes the reaction of carbovir diphosphate and ATP to form carbovir triphosphate and ADP. The activity of human PCK1 and relative inactivity of human nucleoside diphosphate kinase are inferred from the properties of the purified rat and bovine enzymes in vitro (Miller et al. 1992). Carbovir triphosphate is the active metabolite that inhibits HIV infection by acting both as a competitive inhibitor of the natural deoxynucleoside triphosphates at the reverse transcriptase and as a DNA chain terminator following incorporation of the inhibitor into viral DNA (Faletto et al. 1997).
Literature References
PubMed ID Title Journal Year
1383219 Phosphorylation of carbovir enantiomers by cellular enzymes determines the stereoselectivity of antiviral activity

Miller, WH, Boyd, FL, Miller, RL, Hopkins, S, Garvey, EP, Reardon, JE, Daluge, SM

J Biol Chem 1992
9145876 Unique intracellular activation of the potent anti-human immunodeficiency virus agent 1592U89

Miller, WH, Good, SS, Garvey, EP, St Clair, MH, Daluge, SM, Faletto, MB

Antimicrob Agents Chemother 1997
Participants
Input
Output
Participates
as an event of
Catalyst Activity

nucleoside diphosphate kinase activity of PCK1 [cytosol]

Physical Entity
PCK1 [cytosol] (Homo sapiens)
Activity
nucleoside diphosphate kinase activity (GO:0004550)
Inferred From
carbovir diphosphate + ATP => carbovir triphosphate + ADP (Rattus norvegicus)
Authored
D'Eustachio, P  (2012-03-14)
Reviewed
Jassal, B  (2012-03-16)
Created
D'Eustachio, P  (2012-03-14)
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