Reactome | Condensation of Prophase Chromosomes

Condensation of Prophase Chromosomes

Stable Identifier

R-HSA-2299718

DOI

10.3180/REACT_172744.1

Type

Pathway

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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In mitotic prophase, the action of the condensin II complex enables initial chromosome condensation.

The condensin II complex subunit NCAPD3 binds monomethylated histone H4 (H4K20me1), thereby associating with chromatin (Liu et al. 2010). Binding of the condensin II complex to chromatin is partially controlled by the presence of RB1 (Longworth et al. 2008).

Two mechanisms contribute to the accumulation of H4K20me1 at mitotic entry. First, the activity of SETD8 histone methyltransferase peaks at G2/M transition (Nishioka et al. 2002, Rice et al. 2002, Wu et al. 2010). Second, the complex of CDK1 and cyclin B1 (CDK1:CCNB1) phosphorylates PHF8 histone demethylase at the start of mitosis, removing it from chromatin (Liu et al. 2010).

Condensin II complex needs to be phosphorylated by the CDK1:CCNB1 complex, and then phosphorylated by PLK1, in order to efficiently condense prophase chromosomes (Abe et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
12086618	PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin Nishioka, K, Rice, JC, Sarma, K, Erdjument-Bromage, H, Werner, J, Wang, Y, Chuikov, S, Valenzuela, P, Tempst, P, Steward, R, Lis, JT, Allis, CD, Reinberg, D	Mol. Cell	2002
20966048	Dynamic regulation of the PR-Set7 histone methyltransferase is required for normal cell cycle progression Wu, S, Wang, W, Kong, X, Congdon, LM, Yokomori, K, Kirschner, MW, Rice, JC	Genes Dev.	2010
20622854	PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression Liu, W, Tanasa, B, Tyurina, OV, Zhou, TY, Gassmann, R, Liu, WT, Ohgi, KA, Benner, C, Garcia-Bassets, I, Aggarwal, AK, Desai, A, Dorrestein, PC, Glass, CK, Rosenfeld, MG	Nature	2010
21498573	The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II Abe, S, Nagasaka, K, Hirayama, Y, Kozuka-Hata, H, Oyama, M, Aoyagi, Y, Obuse, C, Hirota, T	Genes Dev.	2011
12208845	Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes Rice, JC, Nishioka, K, Sarma, K, Steward, R, Reinberg, D, Allis, CD	Genes Dev.	2002
18367646	RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3 Longworth, MS, Herr, A, Ji, JY, Dyson, NJ	Genes Dev	2008