GORASP1 phosphorylated by PLK1 and GORASP2 phosphorylated by MAPK3-3/MAPK1 are unable to promote Golgi cisternae stacking

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R-HSA-2314566
Type
Reaction [omitted]
Species
Homo sapiens
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Adjacent cisternae of the Golgi apparatus are stacked and linked by tubules to from a Golgi ribbon (Nakamura et al. 2012). GORASP1 (GRASP65), a protein localizing to membranes of cis-Golgi cisternae, enables stacking by in trans dimerization/oligomerization through its PDZ domains (Tang et al. 2010). In mitosis, GORASP1 is phosphorylated by CDK1 and PLK1 (Preisinger et al. 2005). PLK1-mediated phosphorylation of GORASP1 prevents stacking of Golgi cisternae and contributes to unlinking and fragmentation of the Golgi apparatus, probably by interfering with GORASP1 oligomerization (Wang et al. 2003, Sengupta and Linstedt 2010). Similarly, GORASP2 (GRASP55), localized to median Golgi cisternae, promotes stacking by trans-oligomerization. Trans-oligomerization of GORASP2 is prevented by mitotic phosphorylation of GORASP2 downstream of MEK/ERK cascade, and contributes to the Golgi fragmentation in prophase (Xiang and Wang 2010).

Literature References
PubMed ID Title Journal Year
15678101 Plk1 docking to GRASP65 phosphorylated by Cdk1 suggests a mechanism for Golgi checkpoint signalling

Preisinger, C, Korner, R, Wind, M, Lehmann, WD, Kopajtich, R, Barr, FA

EMBO J 2005
20937827 Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand

Sengupta, D, Linstedt, AD

J. Biol. Chem. 2010
22726585 Modular organization of the mammalian Golgi apparatus

Nakamura, N, Wei, JH, Seemann, J

Curr. Opin. Cell Biol. 2012
20083603 GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking

Xiang, Y, Wang, Y

J. Cell Biol. 2010
12839990 A direct role for GRASP65 as a mitotically regulated Golgi stacking factor

Wang, Y, Seemann, J, Pypaert, M, Shorter, J, Warren, G

EMBO J. 2003
20214750 The role of GRASP65 in Golgi cisternal stacking and cell cycle progression

Tang, D, Yuan, H, Wang, Y

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