CDK1 phosphorylates MASTL

Stable Identifier
R-HSA-2430533
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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At the beginning of mitosis, MASTL (GWL, Greatwall kinase) is activated by phosphorylation at several key sites. Many of these sites, including functionally important threonine residues T194, T207 and T741 (corresponding to Xenopus residues T193, T206 and T748), are proline directed, matching CDK1 consensus sequence, and thus probably phosphorylated by CDK1, as shown by in vitro studies (Yu et al. 2006. Blake-Hodek et al. 2012). Phosphorylation of the serine residue S875 (S883 in Xenopus) is implicated as critical for the mitotic function of MASTL (Vigneron et al. 2011) and likely occurs through autophosphorylation (Blake-Hodek et al. 2012). Other kinases, such as PLK1 (Vigneron et al. 2011) and other MASTL phosphorylation sites may also be involved in mitotic activation of MASTL (Yu et al. 2006, Vigneron et al. 2011, Blake-Hodek et al. 2012). Phosphorylation of the serine residue S102 (S101 in Xenopus) is functionally important but the responsible kinase has not been identified (Blake-Hodek et al. 2012).

Literature References
PubMed ID Title Journal Year
21444715 Characterization of the mechanisms controlling Greatwall activity

Vigneron, S, Gharbi-Ayachi, A, Raymond, AA, Burgess, A, Labbe, JC, Labesse, G, Monsarrat, B, Lorca, T, Castro, A

Mol. Cell. Biol. 2011
16600872 Greatwall kinase participates in the Cdc2 autoregulatory loop in Xenopus egg extracts

Yu, J, Zhao, Y, Li, Z, Galas, S, Goldberg, ML

Mol. Cell 2006
22354989 Determinants for activation of the atypical AGC kinase Greatwall during M phase entry

Blake-Hodek, KA, Williams, BC, Zhao, Y, Castilho, PV, Chen, W, Mao, Y, Yamamoto, TM, Goldberg, ML

Mol. Cell. Biol. 2012
Participants
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Catalyst Activity
Catalyst Activity
Title
cyclin-dependent protein serine/threonine kinase activity of CCNB1:p-T161-CDK1 [nucleoplasm]
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Activity
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Created