Reactome | Phosphorylation of cohesin by PLK1 at chromosomal arms

Phosphorylation of cohesin by PLK1 at chromosomal arms

Stable Identifier

R-HSA-2466068

Type

Reaction [transition]

Species

Homo sapiens

Compartment

chromosome, cytosol

ReviewStatus

4/5

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Phosphorylation of cohesin by PLK1 at chromosomal arms

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Prior to anaphase onset, sister-chromatids are held together by cohesin complexes. PLK1-dependent phosphorylation of the cohesin subunit STAG2 (SA2) (Hauf et al. 2005) promotes dissociation of cohesins from chromosomal arms in prometaphase (Hauf et al. 2001). Besides phosphorylating STAG2, PLK1 also phosphorylates RAD21 cohesin subunit, but the phosphorylation of RAD21 is not required for the dissociation of cohesin from chromosomal arms in early mitosis (Hauf et al. 2005). There are several potential PLK1 phosphorylation sites in STAG2 and RAD21, but the exact positions of in vivo phosphorylation of STAG2 and RAD21 by PLK1 have not been explicitly established (Hauf et al. 2005). It is likely that the phosphorylation of cohesin-bound CDCA5 (Sororin) by CDK1 creates a docking site for PLK1 at threonine T159 of CDCA5, thus enabling PLK1 to phosphorylate cohesin subunits (Zhang et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
11509732	Cohesin cleavage by separase required for anaphase and cytokinesis in human cells Hauf, S, Waizenegger, IC, Peters, JM	Science	2001
21987589	Interaction of Sororin protein with polo-like kinase 1 mediates resolution of chromosomal arm cohesion Zhang, N, Panigrahi, AK, Mao, Q, Pati, D	J. Biol. Chem.	2011
15737063	Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2 Hauf, S, Roitinger, E, Koch, B, Dittrich, CM, Mechtler, K, Peters, JM	PLoS Biol	2005