ESPL1 (Separase) cleaves centromeric cohesin

Stable Identifier
Reaction [transition]
Homo sapiens
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ESPL1 (separin i.e. separase) cleaves RAD21 (SCC1) subunit of centromeric cohesin at two sites that conform to the consensus separase recognition site E-X-X-R: after arginine residue R172 and after arginine residue R450 (Hauf et al. 2001). Phosphorylation of RAD21 at the serine residue S454 by PLK1 in prometaphase facilitates ESPL1-mediated cleavage of RAD21 at the C-terminal cleavage site R450 (Hauf et al. 2005). The N-terminal and C-terminal RAD21 cleavage fragments remain bound to the rest of the cohesin complex (Deardorff et al. 2012). It is not clear whether RAD21 middle fragment also continues to be associated with cohesin.

Literature References
PubMed ID Title Journal Year
11509732 Cohesin cleavage by separase required for anaphase and cytokinesis in human cells

Peters, JM, Hauf, S, Waizenegger, IC

Science 2001
22885700 HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle

Shirahige, K, Kiyono, T, Magnaghi-Jaulin, L, Clark, D, Katou, Y, Krantz, ID, Kaiser, FJ, Takagaki, K, Ernst, S, Nozaki, N, Siu, VM, Prigent, C, Christianson, DW, De Baere, E, Decroos, C, Francey, LJ, Di Donato, N, Bando, M, Saitoh, K, Kaur, M, Hirashima, K, Itoh, T, Suzuki, Y, Nakato, R, Mortier, GR, Seimiya, H, Wilde, JJ, Gillessen-Kaesbach, G, Jackson, LG, Watrin, E, Gyftodimou, Y, Willems, PJ, Lombardi, PM, Petersen, MB, Deardorff, MA, Ishikawa, Y, Minamino, M, Jaulin, C, Cole, KE, Hirota, T, Komata, M, Hullings, M

Nature 2012
15737063 Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2

Roitinger, E, Mechtler, K, Dittrich, CM, Peters, JM, Hauf, S, Koch, B

PLoS Biol 2005
Catalyst Activity

cysteine-type peptidase activity of ESPL1 Autocleaved [cytosol]

Orthologous Events
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