ESPL1 (Separase) cleaves centromeric cohesin

Stable Identifier
R-HSA-2467809
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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ESPL1 (separin i.e. separase) cleaves RAD21 (SCC1) subunit of centromeric cohesin at two sites that conform to the consensus separase recognition site E-X-X-R: after arginine residue R172 and after arginine residue R450 (Hauf et al. 2001). Phosphorylation of RAD21 at the serine residue S454 by PLK1 in prometaphase facilitates ESPL1-mediated cleavage of RAD21 at the C-terminal cleavage site R450 (Hauf et al. 2005). The N-terminal and C-terminal RAD21 cleavage fragments remain bound to the rest of the cohesin complex (Deardorff et al. 2012). It is not clear whether RAD21 middle fragment also continues to be associated with cohesin.

Literature References
PubMed ID Title Journal Year
22885700 HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle

Deardorff, MA, Bando, M, Nakato, R, Watrin, E, Itoh, T, Minamino, M, Saitoh, K, Komata, M, Katou, Y, Clark, D, Cole, KE, De Baere, E, Decroos, C, Di Donato, N, Ernst, S, Francey, LJ, Gyftodimou, Y, Hirashima, K, Hullings, M, Ishikawa, Y, Jaulin, C, Kaur, M, Kiyono, T, Lombardi, PM, Magnaghi-Jaulin, L, Mortier, GR, Nozaki, N, Petersen, MB, Seimiya, H, Siu, VM, Suzuki, Y, Takagaki, K, Wilde, JJ, Willems, PJ, Prigent, C, Gillessen-Kaesbach, G, Christianson, DW, Kaiser, FJ, Jackson, LG, Hirota, T, Krantz, ID, Shirahige, K

Nature 2012
11509732 Cohesin cleavage by separase required for anaphase and cytokinesis in human cells

Hauf, S, Waizenegger, IC, Peters, JM

Science 2001
15737063 Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2

Hauf, S, Roitinger, E, Koch, B, Dittrich, CM, Mechtler, K, Peters, JM

PLoS Biol 2005
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
cysteine-type peptidase activity of ESPL1 Autocleaved [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created