Nuclear Pore Complex (NPC) Disassembly

Stable Identifier
R-HSA-3301854
Type
Pathway
Species
Homo sapiens
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Nuclear envelope breakdown in mitosis involves permeabilization of the nuclear envelope through disassembly of the nuclear pore complex (NPC) (reviewed by Guttinger et al. 2009). Nucleoporin NUP98, located at both the cytoplasmic and the nucleoplasmic side of the NPC (Griffis et al. 2003), and involved in the formation of the transport barrier through its FG (phenylalanine glycine) repeats that protrude into the central cavity of the NPC (Hulsmann et al. 2012), is probably the first nucleoporin that dissociates from the NPC at the start of mitotic NPC disassembly (Dultz et al. 2008). NUP98 dissociation is triggered by phosphorylation. Phosphorylation of NUP98 by CDK1 and NIMA family kinases NEK6 and/or NEK7 is needed for NUP98 dissociation from the NPC (Laurell et al. 2011). While the phosphorylation of NUP98 by CDK1 and NEK6/7 is likely to occur simultaneously, CDK1 and NEK6/7-mediated phosphorylations are shown as separate events, for clarity purposes.

Literature References
PubMed ID Title Journal Year
21335236 Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry

Laurell, E, Beck, K, Krupina, K, Theerthagiri, G, Bodenmiller, B, Horvath, P, Aebersold, R, Antonin, W, Kutay, U

Cell 2011
22901806 The permeability of reconstituted nuclear pores provides direct evidence for the selective phase model

Hülsmann, BB, Labokha, AA, Görlich, D

Cell 2012
19234477 Orchestrating nuclear envelope disassembly and reassembly during mitosis

Güttinger, S, Laurell, E, Kutay, U

Nat. Rev. Mol. Cell Biol. 2009
12589057 Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes

Griffis, ER, Xu, S, Powers, MA

Mol. Biol. Cell 2003
18316408 Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells

Dultz, E, Zanin, E, Wurzenberger, C, Braun, M, Rabut, G, Sironi, L, Ellenberg, J

J. Cell Biol. 2008
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