Reactome: A Curated Pathway Database

XIAP monoubiquinates TLE

Stable Identifier
R-HSA-3322429
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

XIAP has been shown to ubiquitinate all human isoforms of TLE in vitro, likely in the conserved Q domain. Ubiquitination does not appear to affect the stability, localization or tetramerization of TLE; rather ubiquitination affects the interaction with TCF/LEF. Ubiquitinated TLE3 is not able to bind TCF7L2 (TCF4) in vitro and addition of XIAP to TLE3-TCF7L2 complexes promotes the dissociation of TLE from TCF7L2. Although XIAP ubiquitinates TLE in a constitutive manner, XIAP only co-immunoprecipitates with TCF7L2 upon activation of the WNT signalling pathway. These data support a model where XIAP regulates the interaction between TLE and TCF/LEF by limiting the pool of free nuclear TLE that is available for binding, and by potentially disrupting existing repression complexes at WNT-responsive promoters. By disrupting the interaction between TLE and TCF/LEF, XIAP may facilitate the recruitment of beta-catenin and the establishment of an activation complex at WNT-responsive promoters (Hanson et al, 2012)

Literature References
PubMed ID Title Journal Year
Participants
Participant Of
This entity is regulated by:
Title Physical Entity Activity
ubiquitin protein ligase activity of TLE:XIAP [nucleoplasm] TLE:XIAP [nucleoplasm] ubiquitin protein ligase activity (0061630)
Orthologous Events