Tankyrase binds AXIN

Stable Identifier
R-HSA-3640862
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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Several recent chemical screens have identified inhibitors of the poly-ADP ribosylation enzymes tankyrase (TNKS) 1 and 2 as regulators of WNT signalling (Huang et al, 2009; Chen et al, 2009; Waaler et al, 2012). Endogenous TNKS1 and 2 associate with AXIN2 in SW480 cells as assessed by co-immunoprecipitation. Both AXIN1 and AXIN2 interact strongly with TNKS1/2 by two-hybrid, and deletion analysis shows that amino acids 19-30 of AXIN1 are necessary and sufficient for binding to TNKS1. This region, termed the tankyrase-binding-domain (TBD) is necessary and sufficient for the interaction in GST-pulldown and co-immunoprecipitation studies (Huang et al, 2009).

Literature References
PubMed ID Title Journal Year
19759537 Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling

Huang, SM, Mishina, YM, Liu, S, Cheung, A, Stegmeier, F, Michaud, GA, Charlat, O, Wiellette, E, Zhang, Y, Wiessner, S, Hild, M, Shi, X, Wilson, CJ, Mickanin, C, Myer, V, Fazal, A, Tomlinson, R, Serluca, F, Shao, W, Cheng, H, Shultz, M, Rau, C, Schirle, M, Schlegl, J, Ghidelli, S, Fawell, S, Lu, C, Curtis, D, Kirschner, MW, Lengauer, C, Finan, PM, Tallarico, JA, Bouwmeester, T, Porter, JA, Bauer, A, Cong, F

Nature 2009
19125156 Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

Chen, B, Dodge, ME, Tang, W, Lu, J, Ma, Z, Fan, CW, Wei, S, Hao, W, Kilgore, J, Williams, NS, Roth, MG, Amatruda, JF, Chen, C, Lum, L

Nat. Chem. Biol. 2009
22440753 A novel tankyrase inhibitor decreases canonical Wnt signaling in colon carcinoma cells and reduces tumor growth in conditional APC mutant mice

Waaler, J, Machon, O, Tumova, L, Dinh, H, Korinek, V, Wilson, SR, Paulsen, JE, Pedersen, NM, Eide, TJ, Machonova, O, Gradl, D, Voronkov, A, von Kries, JP, Krauss, S

Cancer Res. 2012
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