Phosphorylation of nephrin by FYN

Stable Identifier
R-HSA-373747
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

Nephrin has eight putative phosphorylation sites, which nicely match with substrate sites for the Src kinase family. Nephrin, is tyrosine phosphorylated by the Src family tyrosine kinase, Fyn in developing and injured podocytes. Phosphorylation of three of these tyrosines (1176, 1193 and 1217) results in the formation of a preferred binding motif (YDXV) for the SH2 domain of the adaptor protein NCK, which is one of the bricks linking Nephrin to the cytoskeleton. Phosphorylation of tyrosine 1158 results in a binding motif for the p85 regulatory subunit of PI3K.

Literature References
PubMed ID Title Journal Year
12846735 Clustering-induced tyrosine phosphorylation of nephrin by Src family kinases

Lahdenpera, J, Kilpelainen, P, Liu, XL, Pikkarainen, T, Reponen, P, Ruotsalainen, V, Tryggvason, K

Kidney Int 2003
18033240 Nephrin mediates actin reorganization via phosphoinositide 3-kinase in podocytes

Zhu, J, Sun, N, Aoudjit, L, Li, H, Kawachi, H, Lemay, S, Takano, T

Kidney Int 2008
16543952 Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization

Verma, R, Kovari, I, Soofi, A, Nihalani, D, Patrie, K, Holzman, LB

J Clin Invest 2006
15579503 SRC-family kinase Fyn phosphorylates the cytoplasmic domain of nephrin and modulates its interaction with podocin

Li, H, Lemay, S, Aoudjit, L, Kawachi, H, Takano, T

J Am Soc Nephrol 2004
12668668 Fyn binds to and phosphorylates the kidney slit diaphragm component Nephrin

Verma, R, Wharram, B, Kovari, I, Kunkel, R, Nihalani, D, Wary, KK, Wiggins, RC, Killen, P, Holzman, LB

J Biol Chem 2003
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein tyrosine kinase activity of NPHS1 dimer:KIRREL:FYN [plasma membrane]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed