Ena/VASP proteins (ENAH, EVL and VASP) are required in part for ROBO's repulsive output. Ena/VASP proteins are drawn as effectors downstream of Robo signaling via a direct interaction with ROBO. ROBO's CC2 (LPPPP) motif is the consensus binding site for the EVH1 domain of Ena/VASP proteins.
The Ena/VASP family of proteins has a universal role in control of cell motility and actin dynamics. These proteins consist of an N-terminal EVH1 domain, a central proline rich region, which acts as a ligand for the actin monomer binding protein Profilin (PFN), as well as several SH3 domain-containing proteins, such as ABL, and a C-terminal EVH2 domain involved in oligomerization and F-actin binding (Bashaw et al. 2000).