isocitrate + NADP+ => 2-oxoglutarate + CO2 + NADPH + H+

Stable Identifier
R-HSA-389540
Type
Reaction [transition]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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Cytosolic IDH1 (isocitrate dehydrogenase 1) homodimer catalyzes the reaction of isocitrate and NADP+ to form 2-oxoglutarate, CO2, and NADPH + H+. The same enzyme can also localize to peroxisomes (Geisbrecht and Gould 1999; Xu et al. 2004). IDH1 doesn't seem to be part of a hypothetical cytosolic TCA cycle because its product 2-OG would have to be reimported into the mitochondrial matrix. However, the only known transporter of 2-OG, SLC25A12, mainly exports it in exchange with oxoadipate under normal physiological conditions (Fiermonte et al., 2001).
Literature References
PubMed ID Title Journal Year
10521434 The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase

Geisbrecht, BV, Gould, SJ

J Biol Chem 1999
15173171 Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity

Ding, J, Xu, X, Arnold, E, Huang, Q, Xu, Z, Zhao, J, Peng, B

J Biol Chem 2004
11083877 Identification of the human mitochondrial oxodicarboxylate carrier. Bacterial expression, reconstitution, functional characterization, tissue distribution, and chromosomal location

Fiermonte, G, Dolce, V, Ventura, M, Walker, JE, Runswick, MJ, Palmieri, L, Palmieri, F

J Biol Chem 2001
Participants
Participates
Catalyst Activity

isocitrate dehydrogenase (NADP+) activity of IDH1 dimer [cytosol]

Orthologous Events
Cross References
Rhea
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