CERT1-2 releases its bound ceramide into the membrane of the Golgi apparatus

Stable Identifier
R-HSA-429683
Type
Reaction [dissociation]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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CERT1-2 (ceramide transfer protein, isoform 2), associated with the cytosolic face of the endoplasmic reticulum (ER) in a complex with VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006) and PPM1L (protein phosphatase 1-like) (Saito et al. 2008), can bridge the gap between the ER and the Golgi apparatus via its PH domain and transfer a molecule of ceramide extracted from the ER membrane to the Golgi at the ER-Golgi membrane contact sites (MCS) (Hanada et al. 2003; Saito et al. 2008). CERT1-2-mediated ceramide transfer is positively regulated by OSBP (oxysterol binding protein), apparently through accumulation of phosphatidylinositol 4-phosphate (PI-4P) at MCS (Perry and Ridgway 2006; Goto et al., 2016). Non-vesicular transport of ceramide from endoplasmic reticulum to Golgi membranes is essential for cellular lipid homeostasis (reviewed by Olaiyoye et al., 2012; Kumagai & Hanada, 2019).
Literature References
PubMed ID Title Journal Year
16571669 Oxysterol-binding protein and vesicle-associated membrane protein-associated protein are required for sterol-dependent activation of the ceramide transport protein

Ridgway, ND, Perry, RJ

Mol Biol Cell 2006
18165232 Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes

Tamura, S, Saito, S, Kobayashi, T, Echigo, S, Kawano, M, Matsui, H, Hanada, K, Tomishige, N, Kumagai, K

J Biol Chem 2008
14685229 Molecular machinery for non-vesicular trafficking of ceramide

Yasuda, S, Miura, Y, Kawano, M, Fukasawa, M, Kumagai, K, Hanada, K, Nishijima, M

Nature 2003
26601944 Oxysterol-binding Protein Activation at Endoplasmic Reticulum-Golgi Contact Sites Reorganizes Phosphatidylinositol 4-Phosphate Pools

Goto, A, Charman, M, Ridgway, ND

J Biol Chem 2016
31254361 Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites

Hanada, K, Kumagai, K

FEBS Lett 2019
22226883 Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network

Hausser, A, Olayioye, MA

Biochim Biophys Acta 2012
16895911 Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT

Kawano, M, Hanada, K, Kumagai, K, Nishijima, M

J Biol Chem 2006
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