NoRC:HDAC:DNMT methylates cytosine of the rRNA genes

Stable Identifier
R-HSA-5227490
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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From research with human cells (Majumder et al. 2006, Espada et al. 2007) and inferences from mouse cell models, cytosine residues in the main promoter of silenced rRNA gene copiess are methylated by DNMT1 and DNMT3B. DNMT3B directly binds a triple helix formed by pRNA and the main promoter of rDNA. The methylated cytosines prevent binding of the UBF transcription factor, thus preventing transcription of silenced rRNA gene copies. Histone deacetylation is required for DNA methylation.

Literature References
PubMed ID Title Journal Year
17355984 Epigenetic disruption of ribosomal RNA genes and nucleolar architecture in DNA methyltransferase 1 (Dnmt1) deficient cells

Espada, J, Ballestar, E, Santoro, R, Fraga, MF, Villar-Garea, A, Németh, A, Lopez-Serra, L, Ropero, S, Aranda, A, Orozco, H, Moreno, V, Juarranz, A, Stockert, JC, Längst, G, Grummt, I, Bickmore, W, Esteller, M

Nucleic Acids Res 2007
16735507 Role of DNA methyltransferases in regulation of human ribosomal RNA gene transcription

Majumder, S, Ghoshal, K, Datta, J, Smith, DS, Bai, S, Jacob, ST

J. Biol. Chem. 2006
Participants
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Catalyst Activity
Title
DNA-methyltransferase activity of NoRC:pRNA:HDAC:DNMT [nucleoplasm]
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