botE HC:LC binds SV2A or B and GT1b on the target cell surface

Stable Identifier
R-HSA-5244503
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
extracellular region, plasma membrane
ReviewStatus
5/5
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botE HC:LC binds SV2A or B and GT1b on the target cell surface
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The Botulinum toxin type E disulfide bonded heavy chain - light chain heterodimer (botE HC:LC, encoded by the C. botulinum botE gene) (Kumaran et al. 2009) binds ganglioside GT1b and synaptic vesicle protein 2A (SV2A) or 2B (SV2B) on the plasma membrane of a human target cell. In vivo, this process specifically targets synapses at neuromuscular junctions, where toxin association with ganglioside may position it to bind efficiently to SV2A or B when those proteins are exposed at the cell surface by exocytosis (Dong et al. 2008; Rummel et al. 2009).
Literature References
PubMed ID Title Journal Year
19650874 Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor

Rummel, A, Häfner, K, Mahrhold, S, Darashchonak, N, Holt, M, Jahn, R, Beermann, S, Karnath, T, Bigalke, H, Binz, T

J. Neurochem. 2009
19118561 Domain organization in Clostridium botulinum neurotoxin type E is unique: its implication in faster translocation

Kumaran, D, Eswaramoorthy, S, Furey, WF, Navaza, J, Sax, M, Swaminathan, S

J. Mol. Biol. 2009
18815274 Glycosylated SV2A and SV2B mediate the entry of botulinum neurotoxin E into neurons

Dong, M, Liu, H, Tepp, WH, Johnson, EA, Janz, R, Chapman, ER

Mol. Biol. Cell 2008
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Cross References
Mondo
Authored
Krupa, S  (2006-06-15)
Gopinathrao, G  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
D'Eustachio, P  (2014-01-19)
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