botD LC cleaves target cell VAMP2

Stable Identifier
R-HSA-5250606
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
cytosol, synaptic vesicle membrane
ReviewStatus
5/5
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botD LC cleaves target cell VAMP2
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Botulinum toxin type D light chain (botD LC), in the cytosol of a target cell, catalyzes the removal of an aminoterminal peptide from vesicle-associated membrane protein 2 (VAMP2). botD LC is a zinc metalloprotease (Arndt et al. 2006; Schiavo et al. 1993; Yamasaki et al. 1994). VAMP2 is associated with the cytosolic face of the target cell synaptic vesicle and is required for vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release (Sudhof et al, 1993; Sudhof 2004).
Literature References
PubMed ID Title Journal Year
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, De Camilli, P, Niemann, H, Jahn, R

Cell 1993
8226912 Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E

Schiavo, G, Rossetto, O, Catsicas, S, Polverino de Laureto, P, DasGupta, BR, Benfenati, F, Montecucco, C

J. Biol. Chem. 1993
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
16519520 Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity.

Arndt, JW, Chai, Q, Christian, T, Stevens, RC

Biochemistry 2006
8175689 Cleavage of members of the synaptobrevin/VAMP family by types D and F botulinal neurotoxins and tetanus toxin

Yamasaki, S, Baumeister, A, Binz, T, Blasi, J, Link, E, Cornille, F, Roques, B, Fykse, EM, Südhof, TC, Jahn, R

J Biol Chem 1994
Participants
Input
Output
Participates
as an event of
Catalyst Activity

metalloendopeptidase activity of botD LC:Zn2+ [cytosol]

Physical Entity
botD LC:Zn2+ [cytosol] (Clostridium botulinum)
Activity
metalloendopeptidase activity (GO:0004222)
Disease
Name Identifier Synonyms
botulism DOID:11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Cross References
Mondo
Authored
Krupa, S  (2006-06-15)
Gopinathrao, G  (2006-06-15)
Reviewed
Ichtchenko, K  (2007-08-03)
Thirunavukkarasu, N  (2014-11-18)
Sharma, S  (2014-11-18)
Created
D'Eustachio, P  (2014-01-29)
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