BoNT/D LC cleaves target cell VAMP2

Stable Identifier
R-HSA-5250606
Type
Reaction [transition]
Species
Homo sapiens
Related Species
Clostridium botulinum
Compartment
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Botulinum toxin type D light chain (BoNT/D LC), in the cytosol of a target cell, catalyzes the removal of an aminoterminal peptide from vesicle-associated membrane protein 2 (VAMP2). BoNT/D LC is a zinc metalloprotease (Arndt et al. 2006; Schiavo et al. 1993; Yamasaki et al. 1994). VAMP2 is associated with the cytosolic face of the target cell synaptic vesicle and is required for vesicle docking and exocytosis. Its cleavage by botulinum toxin blocks synaptic vesicle fusion with the plasma membrane and neurotransmitter release (Sudhof et al, 1993; Sudhof 2004).

Literature References
PubMed ID Title Journal Year
8226912 Identification of the nerve terminal targets of botulinum neurotoxin serotypes A, D, and E

Schiavo, G, Rossetto, O, Catsicas, S, Polverino de Laureto, P, DasGupta, BR, Benfenati, F, Montecucco, C

J. Biol. Chem. 1993
8402889 Membrane fusion machinery: insights from synaptic proteins

Südhof, TC, De Camilli, P, Niemann, H, Jahn, R

Cell 1993
15217342 The synaptic vesicle cycle

Südhof, TC

Annu Rev Neurosci 2004
16519520 Structure of botulinum neurotoxin type D light chain at 1.65 A resolution: repercussions for VAMP-2 substrate specificity.

Arndt, JW, Chai, Q, Christian, T, Stevens, RC

Biochemistry 2006
8175689 Cleavage of members of the synaptobrevin/VAMP family by types D and F botulinal neurotoxins and tetanus toxin

Yamasaki, S, Baumeister, A, Binz, T, Blasi, J, Link, E, Cornille, F, Roques, B, Fykse, EM, Südhof, TC, Jahn, R

J Biol Chem 1994
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
metalloendopeptidase activity of BoNT/D LC:Zn2+ [cytosol]
Physical Entity
Activity
Disease
Name Identifier Synonyms
botulism 11976 Botulism (disorder), Intoxication with Clostridium botulinum toxin, Botulism, Infection due to clostridium botulinum, Botulism, Foodborne botulism, botulism, Botulism, Botulism, Botulism, Food poisoning due to Clostridium botulinum, Botulism poisoning
Authored
Reviewed
Created