There will be a maintenance on OICR IT systems starting Friday Dec 15 at 6PM EST until midnight Sunday Dec 17; Reactome will be affected by this downtime

Service under maintenance from Friday Dec 15 at 6PM EST until midnight Sunday Dec 17

DNMT3B:DNMT3L methylates cytosine in DNA

Stable Identifier
R-HSA-5334097
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

DNMT3B methylates the 5 position of cytosine in DNA. DNMT3B preferentially methylates cytosine residues that have T at the -1 position and G at the +1 position (Wienholz et al. 2010). Sites methylated de novo by DNMT3B tend to be in transcriptionally inactive regions associated with histone H3 trimethylated at lysine-27 (Choi et al. 2011). Association with DNMT3L increases the processivity of DNMT3B (Van Emburgh and Robertson 2011) and increases methylation at sites that would have low methylation by the activity of DNMT3B alone (Wienholz et al. 2010). Interaction of DNMT3L with DNMT3B-isoform2 stimulates methylation activity significantly (Van Emburgh and Robertson 2011), but interaction of DNMT3L with DNMT3B-isoform1 has little effect on methylation activity (Chedin et al. 2002, Van Emburgh and Robertson 2011). Methylation in embryos but not in gametes (oocytes and spermatozoa) requires DNMT3B (Okano et al. 1999, Kaneda et al. 2004, Borgel et al. 2010, Kaneda et al. 2010).

Participants
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates of DNMT3B:DNMT3L:Chromatin [nucleoplasm]
Physical Entity
Activity
Inferred From