DNMT3B:DNMT3L methylates cytosine in DNA

Stable Identifier
Homo sapiens
Locations in the PathwayBrowser

DNMT3B methylates the 5 position of cytosine in DNA. DNMT3B preferentially methylates cytosine residues that have T at the -1 position and G at the +1 position (Wienholz et al. 2010). Sites methylated de novo by DNMT3B tend to be in transcriptionally inactive regions associated with histone H3 trimethylated at lysine-27 (Choi et al. 2011). Association with DNMT3L increases the processivity of DNMT3B (Van Emburgh and Robertson 2011) and increases methylation at sites that would have low methylation by the activity of DNMT3B alone (Wienholz et al. 2010). Interaction of DNMT3L with DNMT3B-isoform2 stimulates methylation activity significantly (Van Emburgh and Robertson 2011), but interaction of DNMT3L with DNMT3B-isoform1 has little effect on methylation activity (Chedin et al. 2002, Van Emburgh and Robertson 2011). Methylation in embryos but not in gametes (oocytes and spermatozoa) requires DNMT3B (Okano et al. 1999, Kaneda et al. 2004, Borgel et al. 2010, Kaneda et al. 2010).

Literature References
PubMed ID Title Journal Year
20838592 DNMT3L modulates significant and distinct flanking sequence preference for DNA methylation by DNMT3A and DNMT3B in vivo

Wienholz, BL, Kareta, MS, Moarefi, AH, Gordon, CA, Ginno, PA, Chédin, F

PLoS Genet. 2010
15861382 Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family

Chen, ZX, Mann, JR, Hsieh, CL, Riggs, AD, Chédin, F

J. Cell. Biochem. 2005
12481029 The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a

Chédin, F, Lieber, MR, Hsieh, CL

Proc. Natl. Acad. Sci. U.S.A. 2002
21378119 Modulation of Dnmt3b function in vitro by interactions with Dnmt3L, Dnmt3a and Dnmt3b splice variants

Van Emburgh, BO, Robertson, KD

Nucleic Acids Res. 2011
Participant Of
Catalyst Activity
Catalyst Activity
DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates of DNMT3B:DNMT3L:Chromatin [nucleoplasm]
Physical Entity
Inferred From