DNMT3A:DNMT3L binds chromatin

Stable Identifier
Homo sapiens
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DNMT3L is a non-catalytic homologue of DNMT3A and DNMT3B which binds unmethylated lysine-4 of histone H3 (H3K4, Ooi et al. 2007) and recruits DNMT3A-isoform2 (germ cell specific) (Chen et al. 2005, Kareta et al. 2006, Ooi et al. 2007) and, to a lesser extent, other isoforms of DNMT3A via an interaction between the C-terminal regions of DNMT3L and DNMT3A (Chen et al. 2005). Furthermore, DNMT3A binds unmodified tails of histone H3 (Otani et al. 2009). DNMT3L and DNMT3A form tetramers (DNMT3L:DNMT3A:DNMT3A:DNMT3L) that bind DNA located in euchromatic regions (Holz-Schietinger et al. 2011, also inferred from mouse). DNMT3A without DNMT3L forms oligomers that are located in heterochromatin.

Literature References
PubMed ID Title Journal Year
19834512 Structural basis for recognition of H3K4 methylation status by the DNA methyltransferase 3A ATRX-DNMT3-DNMT3L domain

Otani, J, Nankumo, T, Arita, K, Inamoto, S, Ariyoshi, M, Shirakawa, M

EMBO Rep. 2009
15861382 Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family

Chen, ZX, Mann, JR, Hsieh, CL, Riggs, AD, Chédin, F

J. Cell. Biochem. 2005
16829525 Reconstitution and mechanism of the stimulation of de novo methylation by human DNMT3L

Kareta, MS, Botello, ZM, Ennis, JJ, Chou, C, Chédin, F

J. Biol. Chem. 2006
21979949 Oligomerization of DNMT3A controls the mechanism of de novo DNA methylation

Holz-Schietinger, C, Matje, DM, Harrison, MF, Reich, NO

J. Biol. Chem. 2011
17687327 DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA

Ooi, SK, Qiu, C, Bernstein, E, Li, K, Jia, D, Yang, Z, Erdjument-Bromage, H, Tempst, P, Lin, SP, Allis, CD, Cheng, X, Bestor, TH

Nature 2007
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