Reactome | DT A:B binds HBEGF and CD9 on the target cell surface

DT A:B binds HBEGF and CD9 on the target cell surface

Stable Identifier

R-HSA-5336417

Type

Reaction [binding]

Species

Homo sapiens

Related Species

Corynephage beta

Compartment

extracellular region, plasma membrane

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

DT A:B binds HBEGF and CD9 on the target cell surface

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Diphtheria toxin fragments A and B, linked by a disulfide bond (DT A:B) (Collier and Kandel 1971; DeLange et al. 1979; Lambotte et al. 1980; Michel et al. 1972) bind to molecules of proheparin-binding EGF-like growth factor (HBEGF) and CD9 antigen on the target cell plasma membrane. While binding to HBEGF is sufficient for DT A:B uptake into a target cell, presence of CD9 on the target cell surface substantially increases its sensitivity to DT and cross-linking and immunopreciptitation studies indicate that DT A:B, HBEGF, and CD9 form a complex on the cell surface (Brown et al. 1993; Iwamoto et al. 1994). The organization and order of assembly of the complex are not known.

Literature References

PubMed ID	Title	Journal	Year
8194524	Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin sensitivity Iwamoto, R, Higashiyama, S, Mitamura, T, Taniguchi, N, Klagsbrun, M, Mekada, E	EMBO J.	1994
5545092	Structure and activity of diphtheria toxin. I. Thiol-dependent dissociation of a fraction of toxin into enzymically active and inactive fragments Collier, RJ, Kandel, J	J. Biol. Chem.	1971
8367482	Hypersensitivity to diphtheria toxin by mouse cells expressing both diphtheria toxin receptor and CD9 antigen Brown, JG, Almond, BD, Naglich, JG, Eidels, L	Proc. Natl. Acad. Sci. U.S.A.	1993
7462325	Primary structure of diphtheria toxin fragment B: structural similarities with lipid-binding domains Lambotte, P, Falmagne, P, Capiau, C, Zanen, J, Ruysschaert, JM, Dirkx, J	J. Cell Biol.	1980
221484	The amino acid sequence of fragment A, an enzymically active fragment of diphtheria toxin. III. The chymotryptic peptides, the peptides derived by cleavage at tryptophan residues, and the complete sequence of the protein DeLange, RJ, Williams, LC, Drazin, RE, Collier, RJ	J. Biol. Chem.	1979
5022429	Partial characterization of diphtheria toxin and its subunits Michel, A, Zanen, J, Monier, C, Crispeels, C, Dirkx, J	Biochim. Biophys. Acta	1972

Participants

Input

Output

DT:HBEGF:CD9 [plasma membrane] (Homo sapiens)

Participant Of

hasEvent

Uptake and function of diphtheria toxin

Disease

Name	Identifier	Synonyms
diphtheria	11405	corynebacterium infection

Authored

D'Eustachio, P (2014-03-04)

Reviewed

Liu, S (2014-11-18)

Created

D'Eustachio, P (2014-03-02)