DPH6 ligates ammonium to diphthine-EEF2

Stable Identifier
R-HSA-5358475
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Cytosolic diphthamide biosynthesis protein 6 (DPH6) ligates an ammonium ion to diphthine-EEF2 to generate diphthamide-EEF2 in a reaction coupled to the hydrolysis of ATP to yield AMP and PPi (Su et al. 2012; Uthman et al. 2013; Wei et al. 2013).

Literature References
PubMed ID Title Journal Year
23169644 Chemogenomic approach identified yeast YLR143W as diphthamide synthetase

Su, X, Lin, Z, Chen, W, Jiang, H, Zhang, S, Lin, H

Proc. Natl. Acad. Sci. U.S.A. 2012
23468660 The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network

Uthman, S, Bär, C, Scheidt, V, Liu, S, ten Have, S, Giorgini, F, Stark, MJ, Schaffrath, R

PLoS Genet. 2013
23486472 A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene

Wei, H, Bera, TK, Wayne, AS, Xiang, L, Colantonio, S, Chertov, O, Pastan, I

J. Biol. Chem. 2013
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
diphthine-ammonia ligase activity of DPH6 [cytosol]
Physical Entity
Activity
Orthologous Events
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Created