DNA ligase I ligates single stranded nick in double stranded DNA

Stable Identifier
R-HSA-5358592
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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LIG1 (DNA ligase I) catalyzes the formation of a phosphodiester bond between a 3' hydroxyl group and a 5' phosphate group to form an intact DNA strand (Bhat and Grossman 1986). ATP is hydrolyzed to AMP and pyrophosphate in a reaction mechanism that involves adenylylation of a lysine residue of the enzyme, transfer of the adenylyl group to the 5' phosphate of DNA, and nick sealing by nucleophilic attack of the 3' hydroxyl group on the adenylylated 5' phosphate (Taylor et al. 2011, reviewed in Tomkinson et al. 2006). RPA stimulates the activity of LIG1 about 15-fold (Ranalli et al. 2002). LIG1 is recruited to repair sites by interaction with PCNA (Mortusewicz et al. 2006). Ligation is the final step in mismatch repair (Zhang et al. 2005, Constantin et al. 2005).

Literature References
PubMed ID Title Journal Year
16464020 DNA ligases: structure, reaction mechanism, and function

Tomkinson, AE, Vijayakumar, S, Pascal, JM, Ellenberger, T

Chem. Rev. 2006
21561855 Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency

Taylor, MR, Conrad, JA, Wahl, D, O'Brien, PJ

J. Biol. Chem. 2011
16143102 Reconstitution of 5'-directed human mismatch repair in a purified system

Zhang, Y, Yuan, F, Presnell, SR, Tian, K, Gao, Y, Tomkinson, AE, Gu, L, Li, GM

Cell 2005
11698410 Mechanism underlying replication protein a stimulation of DNA ligase I

Ranalli, TA, DeMott, MS, Bambara, RA

J. Biol. Chem. 2002
3004351 Purification and properties of two DNA ligases from human placenta

Bhat, R, Grossman, L

Arch. Biochem. Biophys. 1986
16188885 Human mismatch repair: reconstitution of a nick-directed bidirectional reaction

Constantin, N, Dzantiev, L, Kadyrov, FA, Modrich, P

J. Biol. Chem. 2005
16855289 Differential recruitment of DNA Ligase I and III to DNA repair sites

Mortusewicz, O, Rothbauer, U, Cardoso, MC, Leonhardt, H

Nucleic Acids Res. 2006
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
DNA ligase activity of LIG1 [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created