EXO1 exonucleolytically degrades the strand being repaired in a 5' to 3' direction (Zhang et al. 2005, Orans et al. 2011, and inferred from EXO1 activity with MSH2:MSH6) to create a single -stranded gap extending 90-170 nucleotides beyond the insertion/deletion loop (IDL) (Fang and Modrich 1993). MLH1:PMS2 limits the length of excisions by EXO1 (Zhang et al. 2005). EXO1 also forms a complex with PCNA during S phase (Liberti et al. 2011). RPA binds the resulting single-stranded DNA (Lin et al. 1998, Ramilo et al. 2002, Zhang et al. 2005, reviewed in Iftode et al. 1999).
Fang, WH, Modrich, P
Liberti, SE, Andersen, SD, Wang, J, May, A, Miron, S, Perderiset, M, Keijzers, G, Nielsen, FC, Charbonnier, JB, Bohr, VA, Rasmussen, LJ
Iftode, C, Daniely, Y, Borowiec, JA
Zhang, Y, Yuan, F, Presnell, SR, Tian, K, Gao, Y, Tomkinson, AE, Gu, L, Li, GM
Lin, YL, Shivji, MK, Chen, C, Kolodner, R, Wood, RD, Dutta, A
Ramilo, C, Gu, L, Guo, S, Zhang, X, Patrick, SM, Turchi, JJ, Li, GM
Orans, J, McSweeney, EA, Iyer, RR, Hast, MA, Hellinga, HW, Modrich, P, Beese, LS
5'-3' exonuclease activity of EXO1:MLH1:PMS2:MSH2:MSH3:ATP:PCNA:DNA containing IDL of 2 or more bases and single strand incision [nucleoplasm]
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