Reactome | EXO1 interacting with MSH2:MSH3 excises DNA strand containing an insertion/deletion loop (IDL)

EXO1 interacting with MSH2:MSH3 excises DNA strand containing an insertion/deletion loop (IDL)

Stable Identifier

R-HSA-5358619

Type

Reaction [BlackBoxEvent]

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

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Summation

EXO1 exonucleolytically degrades the strand being repaired in a 5' to 3' direction (Zhang et al. 2005, Orans et al. 2011, and inferred from EXO1 activity with MSH2:MSH6) to create a single -stranded gap extending 90-170 nucleotides beyond the insertion/deletion loop (IDL) (Fang and Modrich 1993). MLH1:PMS2 limits the length of excisions by EXO1 (Zhang et al. 2005). EXO1 also forms a complex with PCNA during S phase (Liberti et al. 2011). RPA binds the resulting single-stranded DNA (Lin et al. 1998, Ramilo et al. 2002, Zhang et al. 2005, reviewed in Iftode et al. 1999).

Literature References

PubMed ID	Title	Journal	Year
8505312	Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction	J. Biol. Chem.	1993
20970388	Bi-directional routing of DNA mismatch repair protein human exonuclease 1 to replication foci and DNA double strand breaks	DNA Repair (Amst.)	2011
10473346	Replication protein A (RPA): the eukaryotic SSB.	Crit Rev Biochem Mol Biol	1999
16143102	Reconstitution of 5'-directed human mismatch repair in a purified system	Cell	2005
9430682	The evolutionarily conserved zinc finger motif in the largest subunit of human replication protein A is required for DNA replication and mismatch repair but not for nucleotide excision repair	J. Biol. Chem.	1998
11884592	Partial reconstitution of human DNA mismatch repair in vitro: characterization of the role of human replication protein A	Mol. Cell. Biol.	2002
21496642	Structures of human exonuclease 1 DNA complexes suggest a unified mechanism for nuclease family	Cell	2011