MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA

Stable Identifier
R-HSA-5419264
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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MTRF1L (mtRF1a) binds the stop codons UAA and UAG of the mRNA when they are in the A site of the ribosome (Soleimanpour-Lichaei 2007, Nozaki et al. 2008). (The UGA codon is recognized by the tryptophan tRNA in mitochondrial translation.) ICT1 can also bind standard stop codons in the A-site (inferred from pig mitochondrial ribosomes in Akabane et al. 2014). MTRF1 was also thought to play a role in translation termination by recognizing the unconventional termination codons AGA and AGG (Zhang and Spremulli 1998, Young et al. 2010) but frameshifting is now confirmed in the termination mechanism of these codons (Temperley et al. 2010). Structural features of MTRF1 have been reported suggesting it could recognize an empty A-site (Huynen et al. 2012) or UAA and UAG codons (Lind et al. 2013) however there is no direct experimental data to confirm these last two postulates.

Literature References
PubMed ID Title Journal Year
24352605 Codon-reading specificities of mitochondrial release factors and translation termination at non-standard stop codons

Lind, C, Sund, J, Aqvist, J

Nat Commun 2013
20421313 Bioinformatic, structural, and functional analyses support release factor-like MTRF1 as a protein able to decode nonstandard stop codons beginning with adenine in vertebrate mitochondria

Young, DJ, Edgar, CD, Murphy, J, Fredebohm, J, Poole, ES, Tate, WP

RNA 2010
20075246 Hungry codons promote frameshifting in human mitochondrial ribosomes

Temperley, R, Richter, R, Dennerlein, S, Lightowlers, RN, Chrzanowska-Lightowlers, ZM

Science 2010
18429816 HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG

Nozaki, Y, Matsunaga, N, Ishizawa, T, Ueda, T, Takeuchi, N

Genes Cells 2008
9838146 Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor

Zhang, Y, Spremulli, LL

Biochim. Biophys. Acta 1998
22569235 Structure based hypothesis of a mitochondrial ribosome rescue mechanism

Huynen, MA, Duarte, I, Chrzanowska-Lightowlers, ZM, Nabuurs, SB

Biol. Direct 2012
17803939 mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG

Soleimanpour-Lichaei, HR, Kühl, I, Gaisne, M, Passos, JF, Wydro, M, Rorbach, J, Temperley, R, Bonnefoy, N, Tate, W, Lightowlers, R, Chrzanowska-Lightowlers, ZM

Mol. Cell 2007
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