Guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1), which is also known as a receptor for activated protein kinase C (RACK1), interacts with NSMAF (FAN) in vitro as shown by glutathione S-transferase-based coprecipitation assays as well as coimmunoprecipitation experiments using human embryonic kidney 293 (HEK293) cells (Tcherkasowa AE et al. 2002). Confocal laser-scanning microscopy studies suggest that overexpressed NSMAF (FAN) and GNB2L1 (RACK) colocalize at the plasma membrane together with TNFR1 (Tcherkasowa AE et al. 2002). Furthemore, isolation of TNF receptors containing vesicles from TNF-stimulated Jurkat or HeLa cells by help of biotinylated TNF and MACS Streptavidin Microbeadsderived and coupled with immunoblotting assay showed that GNB2L1(RACK1) interacts with TNFR1 (Philipp S et al. 2010). The data suggest that GNB2L1(RACK1) modulates activation of neutral sphingomyelinase (N-SMASE) triggered by TNF.