Reactome | SPOP:CUL3:RBX1 ubiquitinates GLI2,3

SPOP:CUL3:RBX1 ubiquitinates GLI2,3

Stable Identifier

R-HSA-5635856

Type

Reaction

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

Expand all

Summation

The transcriptional activity of full-length activated Ci/GLI proteins is restricted by their rapid ubiquitin-mediated degradation after initiation of Hh signaling (Ohlmeyer et al, 1998; Humke et al, 2010; Tukachinsky et al, 2010; Wen et al, 2010). Ubiquitination of Ci, GLI2 and GLI3 is mediated by the E3 ligase complex SPOP:CUL3:RBX1, which ubiquitinates the transcription factors in a Hh-dependent manner (Zhang et al, 2006; Kent et al, 2006; Zhang et al, 2009; Chen et al, 2009).

Literature References

PubMed ID	Title	Journal	Year
16651542	Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus Kent, D, Bush, EW, Hooper, JE	Development	2006
19955409	Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligase Zhang, Q, Shi, Q, Chen, Y, Yue, T, Li, S, Wang, B, Jiang, J	Proc. Natl. Acad. Sci. U.S.A.	2009
19684112	Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved Chen, MH, Wilson, CW, Li, YJ, Law, KK, Lu, CS, Gacayan, R, Zhang, X, Hui, CC, Chuang, PT	Genes Dev.	2009
20154143	Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation Wen, X, Lai, CK, Evangelista, M, Hongo, JA, de Sauvage, FJ, Scales, SJ	Mol. Cell. Biol.	2010
16740475	A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor Zhang, Q, Zhang, L, Wang, B, Ou, CY, Chien, CT, Jiang, J	Dev Cell	2006
20360384	The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins Humke, EW, Dorn, KV, Milenkovic, L, Scott, MP, Rohatgi, R	Genes Dev.	2010
20956384	A mechanism for vertebrate Hedgehog signaling: recruitment to cilia and dissociation of SuFu-Gli protein complexes Tukachinsky, H, Lopez, LV, Salic, A	J. Cell Biol.	2010
9874371	Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator Ohlmeyer, JT, Kalderon, D	Nature	1998