PNKP hydrolyzes the terminal 3'Pi at the NEIL1,NEIL2-generated single strand break (SSB)

Stable Identifier
R-HSA-5649705
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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PNKP, a bifunctional polynucleotide phosphatase/kinase, acts as a 3' phosphatase to remove the terminal 3' phosphate group (3'Pi) at the single strand break (SSB) generated by NEIL1 or NEIL2 beta/delta lyase activity. The presence of XRCC1 is necessary for the 3'-phosphatase activity of PNKP. The removal of the 3'Pi (generating a 3'-OH) makes the DNA with the 3'-end suitable for extension by the DNA polymerase beta (POLB) (Whitehouse et al. 2001, Wiederhold et al. 2004, Das et al. 2006).

Literature References
PubMed ID Title Journal Year
11163244 XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair

Whitehouse, CJ, Taylor, RM, Thistlethwaite, A, Zhang, H, Karimi-Busheri, F, Lasko, DD, Weinfeld, M, Caldecott, KW

Cell 2001
16982218 NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells

Das, A, Wiederhold, L, Leppard, JB, Kedar, P, Prasad, R, Wang, H, Boldogh, I, Karimi-Busheri, F, Weinfeld, M, Tomkinson, AE, Wilson, SH, Mitra, S, Hazra, TK

DNA Repair (Amst.) 2006
15260972 AP endonuclease-independent DNA base excision repair in human cells

Wiederhold, L, Leppard, JB, Kedar, P, Karimi-Busheri, F, Rasouli-Nia, A, Weinfeld, M, Tomkinson, AE, Izumi, T, Prasad, R, Wilson, SH, Mitra, S, Hazra, TK

Mol. Cell 2004
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
polynucleotide 3'-phosphatase activity of NEIL1,NEIL2:PNKP:POLB:LIG3:XRCC1:SSB(3'Pi)-gap-dsDNA [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created