LIG3:XRCC1 and PNKP bind NEIL1,NEIL2:POLB:SSB(3'Pi)-gap-dsDNA

Stable Identifier
R-HSA-5649726
Type
Reaction
Species
Homo sapiens
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LIG3:XRCC1, a complex of DNA ligase 3 and DNA repair protein XRCC1, is recruited to the single nucleotide gap at the SSB (single strand break) site bound by NEIL1 or NEIL2, and POLB, along with polynucleotide kinase/phosphatase PNKP. A large complex containing NEIL1 or NEIL2, PNKP, POLB, LIG3 and XRCC1 can be co-immunoprecipitated from cells undergoing NEIL1 or NEIL2-mediated base excision repair via single nucleotide replacement. NEIL1 interacts with POLB and LIG3 directly, but not with PNKP or XRCC1 (Wiederhold et al. 2004, Das et al. 2006). PNKP directly binds XRCC1, LIG3 and POLB, and the interaction with XRCC1 is essential for the 3'-phosphatase activity of PNKP (Whitehouse et al. 2001).

Literature References
PubMed ID Title Journal Year
11163244 XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair

Whitehouse, CJ, Taylor, RM, Thistlethwaite, A, Zhang, H, Karimi-Busheri, F, Lasko, DD, Weinfeld, M, Caldecott, KW

Cell 2001
16982218 NEIL2-initiated, APE-independent repair of oxidized bases in DNA: Evidence for a repair complex in human cells

Das, A, Wiederhold, L, Leppard, JB, Kedar, P, Prasad, R, Wang, H, Boldogh, I, Karimi-Busheri, F, Weinfeld, M, Tomkinson, AE, Wilson, SH, Mitra, S, Hazra, TK

DNA Repair (Amst.) 2006
15260972 AP endonuclease-independent DNA base excision repair in human cells

Wiederhold, L, Leppard, JB, Kedar, P, Karimi-Busheri, F, Rasouli-Nia, A, Weinfeld, M, Tomkinson, AE, Izumi, T, Prasad, R, Wilson, SH, Mitra, S, Hazra, TK

Mol. Cell 2004
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