PARP1,PARP2 dimers and FEN1 bind POLB and displace APEX1 from damaged AP site

Stable Identifier
R-HSA-5649873
Type
Reaction [binding]
Species
Homo sapiens
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Homodimers and/or heterodimers of PARP1 and PARP2 bind single strand DNA (ssDNA) ends along with FEN1 (flap endonuclease), forming a ternary complex with POLB (DNA polymerase beta) and simultaneously displacing APEX1 (Prasad et al. 2001, Lavrik et al. 2001, Cistulli et al. 2004). While PARP2 is much less catalytically active than PARP1 in DNA damage-induced poly(ADP-ribosyl) (PAR) synthesis (Shieh et al. 1998, Ame et al. 1999, Fisher et al. 2007), the functional redundancy between PARP1 and PARP2 is probably important. Knockout of both PARP1 and PARP2 homologs is embryonic lethal in mice, while knockout of individual PARPs is not (Menissier de Murcia et al. 2003).

Literature References
PubMed ID Title Journal Year
12727891 Functional interaction between PARP-1 and PARP-2 in chromosome stability and embryonic development in mouse

Ménissier de Murcia, J, Ricoul, M, Tartier, L, Niedergang, C, Huber, A, Dantzer, F, Schreiber, V, Amé, JC, Dierich, A, LeMeur, M, Sabatier, L, Chambon, P, de Murcia, G

EMBO J. 2003
10364231 PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase

Amé, JC, Rolli, V, Schreiber, V, Niedergang, C, Apiou, F, Decker, P, Muller, S, Höger, T, Ménissier-de Murcia, J, de Murcia, G

J. Biol. Chem. 1999
17548475 Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase

Fisher, AE, Hochegger, H, Takeda, S, Caldecott, KW

Mol. Cell. Biol. 2007
11340072 Photoaffinity labeling of mouse fibroblast enzymes by a base excision repair intermediate. Evidence for the role of poly(ADP-ribose) polymerase-1 in DNA repair

Lavrik, OI, Prasad, R, Sobol, RW, Horton, JK, Ackerman, EJ, Wilson, SH

J. Biol. Chem. 2001
9804757 Poly(ADP-ribose) polymerase null mouse cells synthesize ADP-ribose polymers

Shieh, WM, Amé, JC, Wilson, MV, Wang, ZQ, Koh, DW, Jacobson, MK, Jacobson, EL

J. Biol. Chem. 1998
11440997 DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis

Prasad, R, Lavrik, OI, Kim, SJ, Kedar, P, Yang, XP, Vande Berg, BJ, Wilson, SH

J. Biol. Chem. 2001
15135726 AP endonuclease and poly(ADP-ribose) polymerase-1 interact with the same base excision repair intermediate

Cistulli, C, Lavrik, OI, Prasad, R, Hou, E, Wilson, SH

DNA Repair (Amst.) 2004
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