Reactome: A Curated Pathway Database

POLB-mediated DNA strand displacement synthesis

Stable Identifier
R-HSA-5649883
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

DNA polymerase beta (POLB) catalyzes DNA strand displacement synthesis. In this process, 2-10 nucleotides (dNMPs) are incorporated at the 3' end of the single strand break (SSB). Simultaneously, the other broken DNA strand, which contains a 5' oxidatively-damaged AP dideoxyribose phosphate residue (5'ddRP), is displaced as a flap structure. POLB-mediated DNA strand displacement synthesis is facilitated by PARP1 and/or PARP2 dimers, which bind the single strand DNA (ssDNA) ends and FEN1 (flap endonuclease) (Klungland and Lindahl 1997, Prasad et al. 2001, Lavrik et al. 2001, Liu et al. 2005).

Literature References
Participants
Participant Of
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Title Physical Entity Activity
DNA-directed DNA polymerase activity of PARP1,PARP2:FEN1:POLB:SSB(3'dNMP-displaced 5'ddRP)-dsDNA [nucleoplasm] PARP1,PARP2:FEN1:POLB:SSB(3'dNMP-displaced 5'ddRP)-dsDNA [nucleoplasm] DNA-directed DNA polymerase activity (0003887)
Orthologous Events