KIAA0101 double monoubiquitination

Stable Identifier
R-HSA-5655193
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
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KIAA0101 (PAF15) is a PCNA-associated protein expressed during S phase of the cell cycle, under the control of members of the E2F transcription factor family (Chang et al. 2013) and degraded at mitotic exit by the APC:CDH1 complex (Emanuele et al. 2011). KIAA0101 is monoubiquitinated on two lysine residues, K15 and K24, by an unknown ubiquitin ligase. Doubly monoubiquitinated KIAA0101 (PAF15) (MonoUb:K15,K24-KIAA0101) binds PCNA and promotes the switch from translesion DNA synthesis (TLS) polymerase, such as DNA polymerase eta (POLH), to replicative DNA polymerases delta (POLD) or epsilon (POLE). KIAA0101 monoubiquitination thus facilitates termination of TLS and coordinates DNA damage bypass events (Povlsen et al. 2012). UV-induced DNA damage causes removal of MonoUb:K15,K24-KIAA0101 by proteasome-mediated degradation, promoting the switch from replicative DNA polymerase complexes delta (POLD) or epsilon (POLE) to translesion DNA synthesis (TLS) polymerases, such as POLH (DNA polymerase eta) (Povlsen et al. 2012).

Literature References
PubMed ID Title Journal Year
23000965 Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass

Povlsen, LK, Beli, P, Wagner, SA, Poulsen, SL, Sylvestersen, KB, Poulsen, JW, Nielsen, ML, Bekker-Jensen, S, Mailand, N, Choudhary, C

Nat. Cell Biol. 2012
21628590 Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate

Emanuele, MJ, Ciccia, A, Elia, AE, Elledge, SJ

Proc. Natl. Acad. Sci. U.S.A. 2011
23593430 p15(PAF) is an Rb/E2F-regulated S-phase protein essential for DNA synthesis and cell cycle progression

Chang, CN, Feng, MJ, Chen, YL, Yuan, RH, Jeng, YM

PLoS ONE 2013
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