POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template

Stable Identifier
R-HSA-5655835
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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DNA polymerase kappa (POLK) forms a quaternary complex with REV1 and the DNA polymerase zeta (POLZ) heterodimer, composed of REV3L and MAD2L2 (REV7), at DNA damage sites (Ohashi et al. 2009, Wojtaszek et al. 2012a, Wojtaszek et al. 2012b, Xie et al. 2012). POLK simultaneously interacts with the C-terminus of REV1 through its RIR (REV1-interacting region) (Ohashi et al. 2009) and with monoubiquitinated PCNA (Haracska et al. 2002, Bi et al. 2006). POLK requires Mg2+ or Mn2+ for its activity. POLK is more catalytically active in the presence of Mn2+, but exhibits higher fidelity in the presence of Mg2+ (Pence et al. 2012)

Literature References
PubMed ID Title Journal Year
22859295 Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) ?, and Pol ?

Wojtaszek, J, Lee, CJ, D'Souza, S, Minesinger, B, Kim, H, D'Andrea, AD, Walker, GC, Zhou, P

J. Biol. Chem. 2012
23143872 Structural insights into the assembly of human translesion polymerase complexes

Xie, W, Yang, X, Xu, M, Jiang, T

Protein Cell 2012
20952260 Bypass of N²-ethylguanine by human DNA polymerase ?

Pence, MG, Blans, P, Zink, CN, Fishbein, JC, Perrino, FW

DNA Repair (Amst.) 2011
16611994 Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest

Bi, X, Barkley, LR, Slater, DM, Tateishi, S, Yamaizumi, M, Ohmori, H, Vaziri, C

Mol. Cell. Biol. 2006
19170759 Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function

Ohashi, E, Hanafusa, T, Kamei, K, Song, I, Tomida, J, Hashimoto, H, Vaziri, C, Ohmori, H

Genes Cells 2009
22700975 Multifaceted recognition of vertebrate Rev1 by translesion polymerases ? and ?

Wojtaszek, J, Liu, J, D'Souza, S, Wang, S, Xue, Y, Walker, GC, Zhou, P

J. Biol. Chem. 2012
11784855 Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA

Haracska, L, Unk, I, Johnson, RE, Phillips, BB, Hurwitz, J, Prakash, L, Prakash, S

Mol. Cell. Biol. 2002
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