DNA polymerase kappa (POLK) is a Y family DNA polymerase that is most efficient in translesion DNA synthesis (TLS) across oxidation derivatives of DNA bases, such as thymine glycol (Tg) and 8-oxoguanine (OGUA), as well as bulky DNA adducts, such as benzo(a)pyrene diol epoxide guanine adduct (BPDE-G) (Zhang et al. 2000, Fischhaber et al.2002, Avkin et al. 2004, Vasquez-Del Carpio et al. 2009, Yoon et al. 2010, Lior-Hoffmann et al. 2012, Christov et al. 2012, Yoon et al. 2014). POLK carries out TLS by forming a quaternary complex with REV1 and POLZ (REV3L:MAD2L2) at DNA damage sites, where POLK simultaneously binds REV1 and monoubiquitinated PCNA (Ohashi et al. 2009, Haracska, Unk et al. 2002, Bi et al. 2006). POLK and POLZ cooperate in the elongation of nucleotides inserted opposite to lesioned bases by POLK. Similarly to POLZ, POLK has low processivity and is error-prone (Ohashi et al. 2000, Haracska, Prakash et al. 2002, Yoon et al. 2010).