MT3 binds zinc

Stable Identifier
R-HSA-5662606
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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The metallothionein MT3 binds 7 zinc(II) atoms in 2 clusters, one cluster of 4 atoms at the C-terminal alpha domain and one cluster of 3 atoms at the N-terminal beta domain (Eriste et al. 2003, Knipp et al. 2005, Meloni et al. 2007, Meloni et al. 2008, Meloni et al. 2009, Wu et al. 2014). Though MT3 has a lower overall affinity for zinc than MT2A does (Palumaa et al. 2005), MT3 is capable of binding an additional zinc atom with a binding constant of 100 micromolar (Eriste et al. 2003, Palumaa et al. 2005, Meloni et al. 2009). Stoichiometries up to MT3:11Zn2+ have been observed but the MT3:7Zn2+ form is more stable and predominates (Palumaa et al. 2002, Eriste et al. 2003, Palumaa et al.).

Literature References
PubMed ID Title Journal Year
24973778 High level expression, efficient purification, and bioactivity of recombinant human metallothionein 3 (rhMT3) from methylotrophic yeast Pichia pastoris

Wu, Q, Li, B, Wu, F, Yang, L, Li, S, Li, H, Wu, D, Cui, T, Tang, D

Protein Expr. Purif. 2014
15698955 Metal binding of metallothionein-3 versus metallothionein-2: lower affinity and higher plasticity

Palumaa, P, Tammiste, I, Kruusel, K, Kangur, L, Jörnvall, H, Sillard, R

Biochim. Biophys. Acta 2005
15736926 Zn7metallothionein-3 and the synaptic vesicle cycle: interaction of metallothionein-3 with the small GTPase Rab3A

Knipp, M, Meloni, G, Roschitzki, B, Vasák, M

Biochemistry 2005
19425569 Effects of Zn(2+), Ca(2+), and Mg(2+) on the structure of Zn(7)metallothionein-3: evidence for an additional zinc binding site

Meloni, G, Polanski, T, Braun, O, Vasák, M

Biochemistry 2009
17389590 Redox silencing of copper in metal-linked neurodegenerative disorders: reaction of Zn7metallothionein-3 with Cu2+ ions

Meloni, G, Faller, P, Vasák, M

J. Biol. Chem. 2007
18454142 Metal swap between Zn7-metallothionein-3 and amyloid-beta-Cu protects against amyloid-beta toxicity

Meloni, G, Sonois, V, Delaine, T, Guilloreau, L, Gillet, A, Teissié, J, Faller, P, Vasák, M

Nat. Chem. Biol. 2008
11994011 Brain-specific metallothionein-3 has higher metal-binding capacity than ubiquitous metallothioneins and binds metals noncooperatively

Palumaa, P, Eriste, E, Njunkova, O, Pokras, L, Jörnvall, H, Sillard, R

Biochemistry 2002
12963354 Purification of recombinant human apometallothionein-3 and reconstitution with zinc

Eriste, E, Kruusel, K, Palumaa, P, Jörnvall, H, Sillard, R

Protein Expr. Purif. 2003
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