Once activated by binding to RHOA:GTP, DIAPH1 binds profilin:G-actin complexes together with EVL (VASP) homotetramers and promotes elongation of actin filaments (Copeland and Treisman 2002, Grosse et al. 2003, Kursula et al. 2008, Breitsprecher et al. 2008). Binding of nonpolymerized actin (G-actin) to DIAPH1 and EVL releases MKL1 (MAL) transcription co-factor which is inhibited when bound to G-actin (Miralles et al. 2003).