BLM mediates dissolution of double Holliday junction

Stable Identifier
R-HSA-5686410
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The BTRR complex, composed of BLM, TOP3A, RMI1 and RMI2, dissolves double Holliday junctions by disentangling hemicatenane intermediates (Bocquet et al. 2014). This results in non-crossover products, where no exchange of genetic material happens between the sister chromatid that served as a template for the DNA repair synthesis and the repaired DNA duplex. SPIDR serves as a scaffold that connects the BTRR complex with the double Holliday junction through its simultaneous interaction with RAD51-coated DNA strands of the Holliday junction and BLM. SPIDR is needed for BTRR-mediated prevention of cross-over between sister chromatids (Wan et al. 2013).

Literature References
PubMed ID Title Journal Year
24509834 Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III? and RMI1

Bocquet, N, Bizard, AH, Abdulrahman, W, Larsen, NB, Faty, M, Cavadini, S, Bunker, RD, Kowalczykowski, SC, Cejka, P, Hickson, ID, Thomä, NH

Nat. Struct. Mol. Biol. 2014
23509288 Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair

Wan, L, Han, J, Liu, T, Dong, S, Xie, F, Chen, H, Huang, J

Proc. Natl. Acad. Sci. U.S.A. 2013
Participants
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Catalyst Activity
Catalyst Activity
Title
helicase activity of BLM:TOP3A:RMI1:RMI2:SPIDR [nucleoplasm]
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