Reactome | MUS81:EME1,EME2 cleaves D-loop

MUS81:EME1,EME2 cleaves D-loop

Stable Identifier

R-HSA-5686440

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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DNA Repair (Homo sapiens)

- DNA Double-Strand Break Repair (Homo sapiens)
  - Homology Directed Repair (Homo sapiens)
    - HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) (Homo sapiens)
      - HDR through Homologous Recombination (HRR) (Homo sapiens)
        
        Resolution of D-Loop Structures (Homo sapiens)
        
        Resolution of D-loop Structures through Holliday Junction Intermediates (Homo sapiens)
        
        MUS81:EME1,EME2 cleaves D-loop (Homo sapiens)

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The complex of MUS81 and EME1 or EME2 acts as an endonuclease and processes D-loops by cleaving the sister chromatid strand complementary to the invading strand at the junction point. MUS81:EME2 complex has a higher catalytic activity than MUS81:EME1 complex and, in vitro, cleaves D-loops at several other sites in addition to the junction point (Osman et al. 2003, Pepe and West 2014). The yeast Mus81:Eme1 (Mus81:Mms4) complex also acts on D-loops (Schwartz et al. 2012).

Literature References

PubMed ID	Title	Journal	Year
14527420	Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis Doe, CL, Osman, F, Whitby, MC, Dixon, J	Mol. Cell	2003
24371268	Substrate specificity of the MUS81-EME2 structure selective endonuclease West, SC, Pepe, A	Nucleic Acids Res.	2014
22645308	Mus81-Mms4 functions as a single heterodimer to cleave nicked intermediates in recombinational DNA repair Heyer, WD, Wright, WD, Stahlberg, H, Schwartz, EK, Evans, JE, Ehmsen, KT	Mol. Cell. Biol.	2012