ERCC1:XPF cleaves flaps generated by SSA

Stable Identifier
R-HSA-5686657
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The endonuclease complex ERCC1:XPF (ERCC1:ERCC4) is recruited to single strand annealing (SSA) sites of DNA double strand break (DSB) repair through direct interaction between XPF (ERCC4) and RAD52 (Motycka et al. 2004). ERCC1:XPF cleaves the ssDNA flaps generated by displacement of non-complementary 3' parts of 3' ssDNA overhangs during RAD52-mediated annealing. The enzymatic activity of ERCC1:XPF is necessary for the completion of SSA (Motycka et al. 2004, Al-Minawi et al. 2008, Ahmad et al. 2008).

Literature References
PubMed ID Title Journal Year
17962301 The ERCC1/XPF endonuclease is required for efficient single-strand annealing and gene conversion in mammalian cells

Al-Minawi, AZ, Saleh-Gohari, N, Helleday, T

Nucleic Acids Res. 2008
18541667 ERCC1-XPF endonuclease facilitates DNA double-strand break repair

Ahmad, A, Robinson, AR, Duensing, A, van Drunen, E, Beverloo, HB, Weisberg, DB, Hasty, P, Hoeijmakers, JH, Niedernhofer, LJ

Mol. Cell. Biol. 2008
14734547 Physical and functional interaction between the XPF/ERCC1 endonuclease and hRad52

Motycka, TA, Bessho, T, Post, SM, Sung, P, Tomkinson, AE

J. Biol. Chem. 2004
Participants
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Catalyst Activity
Title
endodeoxyribonuclease activity of ERCC1:ERCC4 [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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