3'-incision of DNA by ERCC5 (XPG) in GG-NER

Stable Identifier
R-HSA-5690988
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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In global genome nucleotide excision repair (GG-NER), as well as transcription-coupled nucleotide excision repair (TC-NER), the cleavage of the damaged DNA strand 3' to the site of damage is carried out by a DNA endonuclease XPG (ERCC5). While the DNA repair synthesis may be initiated prior to the 3' incision (Staresincic et al. 2009), the components of the incision complex probably dissociate from the NER site shortly after the replicative complex assembly and 3' incision (Overmeer et al. 2011). The exception is the RPA heterotrimer, which is a constituent of the DNA synthesis complex, and also coats the undamaged DNA strand, thereby protecting it from endonucleolytic cleavage.

Literature References
PubMed ID Title Journal Year
19279666 Coordination of dual incision and repair synthesis in human nucleotide excision repair

Staresincic, L, Fagbemi, AF, Enzlin, JH, Gourdin, AM, Wijgers, N, Dunand-Sauthier, I, Giglia-Mari, G, Clarkson, SG, Vermeulen, W, Schärer, OD

EMBO J. 2009
21282463 Replication protein A safeguards genome integrity by controlling NER incision events

Overmeer, RM, Moser, J, Volker, M, Kool, H, Tomkinson, AE, van Zeeland, AA, Mullenders, LH, Fousteri, M

J. Cell Biol. 2011
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Event Information
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity of GG-NER incision complex:5'-incised damaged DNA:(PCNA:POLD,POLE),(MonoUb:K164-PCNA:POLK):RPA:RFC [nucleoplasm]
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Orthologous Events
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