Initial resection of double-strand break ends

Stable Identifier
R-HSA-5693608
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

In order for repair of the DNA double-strand breaks to occur through homologous recombination or single strand annealing, the 5' ends of the break must first be resected to produce 3' overhanging single stranded DNA (ssDNA) that can subsequently invade homologous duplex DNA (e.g. in the sister chromatid) (Thompson et al. 2001, Kolpashchikov et al. 2001). The MRE11A component of the MRN complex possesses endonuclease activity (Trujillo et al. 1998, Hopfner et al. 2002) that is activated by binding of RBBP8 (CtIP) and BRCA1, in the presence of Mn2+ or Mg2+ ions (Sartori et al. 2007, Yun and Hiom 2009).

Literature References
PubMed ID Title Journal Year
11139606 Polarity of human replication protein A binding to DNA.

Kolpashchikov, DM, Khodyreva, SN, Khlimankov, DY, Wold, MS, Favre, A, Lavrik, OI

Nucleic Acids Res 2001
11376695 Homologous recombinational repair of DNA ensures mammalian chromosome stability

Thompson, LH, Schild, D

Mutat Res 2001
9705271 Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95.

Trujillo, KM, Yuan, SS, Lee, EY, Sung, P

J Biol Chem 1998
17965729 Human CtIP promotes DNA end resection

Sartori, AA, Lukas, C, Coates, J, Mistrik, M, Fu, S, Bartek, J, Baer, R, Lukas, J, Jackson, SP

Nature 2007
19357644 CtIP-BRCA1 modulates the choice of DNA double-strand-break repair pathway throughout the cell cycle

Yun, MH, Hiom, K

Nature 2009
12152085 The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair.

Hopfner, KP, Craig, L, Moncalian, G, Zinkel, RA, Usui, T, Owen, BA, Karcher, A, Henderson, B, Bodmer, JL, McMurray, CT, Carney, JP, Petrini, JH, Tainer, JA

Nature 2002
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity of DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:p-BRCA1-C complex [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed