Presynaptic phase of homologous DNA pairing and strand exchange

Stable Identifier
R-HSA-5693616
Type
Pathway
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

The presynaptic phase of homologous DNA pairing and strand exchange during homologous recombination repair (HRR) begins with the displacement of RPA from ssDNA (Thompson and Limoli 2003) by the joint action of RAD51 and BRCA2. CHEK1-mediated phosphorylation of RAD51 and BRCA2 (Sorensen et al. 2005, Bahassi et al. 2008) is needed for BRCA2-mediated nucleation of RAD51 on 3'-ssDNA overhangs, RPA displacement and formation of RAD51 nucleofilaments (Yang et al. 2005, Jensen et al. 2010, Liu et al. 2010, Thorslund et al. 2010). Invasive RAD51 nucleofilaments are stabilized by the BCDX2 complex composed of RAD51B, RAD51C, RAD51D and XRCC2 (Masson et al. 2001, Chun et al. 2013, Amunugama et al. 2013).

Literature References
PubMed ID Title Journal Year
  Eukaryotic DNA Damage Surveillance and Repair

Caldecott, KW

  2004
20729858 The breast cancer tumor suppressor BRCA2 promotes the specific targeting of RAD51 to single-stranded DNA

Thorslund, T, McIlwraith, MJ, Compton, SA, Lekomtsev, S, Petronczki, M, Griffith, JD, West, SC

Nat. Struct. Mol. Biol. 2010
23149936 Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway

Chun, J, Buechelmaier, ES, Powell, SN

Mol. Cell. Biol. 2013
18317453 The checkpoint kinases Chk1 and Chk2 regulate the functional associations between hBRCA2 and Rad51 in response to DNA damage

Bahassi, EM, Ovesen, JL, Riesenberg, AL, Bernstein, WZ, Hasty, PE, Stambrook, PJ

Oncogene 2008
11751635 Identification and purification of two distinct complexes containing the five RAD51 paralogs

Masson, JY, Tarsounas, MC, Stasiak, AZ, Stasiak, A, Shah, R, McIlwraith, MJ, Benson, FE, West, SC

Genes Dev. 2001
15703751 The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA-ssDNA junction

Yang, H, Li, Q, Fan, J, Holloman, WK, Pavletich, NP

Nature 2005
15665856 The cell-cycle checkpoint kinase Chk1 is required for mammalian homologous recombination repair

Sørensen, CS, Hansen, LT, Dziegielewski, J, Syljuåsen, RG, Lundin, C, Bartek, J, Helleday, T

Nat. Cell Biol. 2005
20729832 Purified human BRCA2 stimulates RAD51-mediated recombination

Jensen, RB, Carreira, A, Kowalczykowski, SC

Nature 2010
23810717 The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament

Amunugama, R, Groden, J, Fishel, R

DNA Repair (Amst.) 2013
20729859 Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA

Liu, J, Doty, T, Gibson, B, Heyer, WD

Nat. Struct. Mol. Biol. 2010
Participants
Participant Of
Orthologous Events
Authored
Reviewed