D-loop formation mediated by PALB2, BRCA2 and RAD51

Stable Identifier
R-HSA-5693620
Type
Reaction
Species
Homo sapiens
Compartment
Synonyms
Strand exchange/Branch migration mediated by PALB2, BRCA2 and RAD51
Locations in the PathwayBrowser
Summation

A D-loop structure is formed when complementary duplex DNA (sister chromatid arm) is progressively invaded by the RAD51 nucleoprotein filament, with base pairing of the invading ssDNA and the complementary sister chromatid DNA strand (Sung et al. 2003). PALB2 and RAD51AP1 synergistically stimulate RAD51 recombinase activity, thus enhancing RAD51-mediated strand exchange (branch migration) and promoting the formation of D-loop structures (synaptic complex assembly). PALB2 simultaneously interacts with RAD51, BRCA2 and RAD51AP1 (Modesti et al. 2007, Wiese et al. 2007, Buisson et al. 2010, Dray et al. 2010). The direct BRCA1 interaction with PALB2 helps to fine-tune the localization of BRCA2 and RAD51 at DNA double strand breaks (DSBs) (Zhang et al. 2009, Sy et al. 2009).

Literature References
PubMed ID Title Journal Year
19268590 PALB2 links BRCA1 and BRCA2 in the DNA-damage response

Zhang, F, Ma, J, Wu, J, Ye, L, Cai, H, Xia, B, Yu, X

Curr. Biol. 2009
20871616 Enhancement of RAD51 recombinase activity by the tumor suppressor PALB2

Dray, E, Etchin, J, Wiese, C, Saro, D, Williams, GJ, Hammel, M, Yu, X, Galkin, VE, Liu, D, Tsai, MS, Sy, SM, Schild, D, Egelman, E, Chen, J, Sung, P

Nat. Struct. Mol. Biol. 2010
19369211 PALB2 is an integral component of the BRCA complex required for homologous recombination repair

Sy, SM, Huen, MS, Chen, J

Proc Natl Acad Sci U S A 2009
17996711 Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement

Wiese, C, Dray, E, Groesser, T, San Filippo, J, Shi, I, Collins, DW, Tsai, MS, Williams, GJ, Rydberg, B, Sung, P, Schild, D

Mol. Cell 2007
12912992 Rad51 recombinase and recombination mediators.

Sung, P, Krejci, L, Van Komen, S, Sehorn, MG

J Biol Chem 2003
20871615 Cooperation of breast cancer proteins PALB2 and piccolo BRCA2 in stimulating homologous recombination

Buisson, R, Dion-Côté, AM, Coulombe, Y, Launay, H, Cai, H, Stasiak, AZ, Stasiak, A, Xia, B, Masson, JY

Nat. Struct. Mol. Biol. 2010
17996710 RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination

Modesti, M, Budzowska, M, Baldeyron, C, Demmers, JA, Ghirlando, R, Kanaar, R

Mol. Cell 2007
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